Пцр тест на английском языке как пишется


На основании Вашего запроса эти примеры могут содержать грубую лексику.


На основании Вашего запроса эти примеры могут содержать разговорную лексику.

Перевод «ПЦР тест» на английский


Забронируй процедурную. проведите эндоскопию верхней части тела и ПЦР тест на болезнь Уиппла.



Book a procedure room, do an upper endoscopy, and run a PCR test for Whipple’s.


Ложный положительный ПЦР тест на СПИД?

Другие результаты


Ожидается, что коммерческая стоимость будет ниже стоимости обычных ПЦР-тестов.


ПЦР-тесты могут обнаружить ДНК червя нематоды.



PCR tests can detect the DNA of the nematode worm.


ПЦР-тесты могут выявлять и идентифицировать патогенные организмы у пациентов, особенно те, которые трудно культивировать.



For example, PCR tests can detect and identify pathogenic organisms in patients, especially those that are difficult to cultivate.


Пациенты, получающие лечение, также могут контролироваться с помощью ПЦР-тестов.



Patients receiving treatment may also be monitored with PCR tests.


Однако такие ПЦР-тесты не доступны на месте, и, таким образом, образец должен быть доставлен во внешнюю лабораторию.



However, these PCR tests are not available on site, and thus the sample must be delivered to the off-site laboratory.


Для этого понадобится выполнить ПЦР-тест и, возможно, провести биопсию.



In this case, you will have to undergo a medical examination and maybe a biopsy.


Результаты вернутся в течение дня после ПЦР-теста, но дольше с мокротой.



Results will come back within a day of the PCR test, but longer with the culture.


Наиболее точный метод исследования является ПЦР-тест.



The most effective test is a CT scan.


Если симптомы болезни Лайма не проходят даже после приема антибиотиков, то пациенту могут предложить пройти ПЦР-тест.



If your symptoms of Lyme disease have not subsided even after taking antibiotics for the same, you may be asked to undergo a PCR test.


разработка иммунологических и ПЦР тест-систем для диагностики инфекционных и инвазионных болезней растений, животных и человека;



plants, animals and humans immunological and PCR test systems development for infectious and invasive diseases diagnosis;


«ПЦР-тест для детекции и дифференциальной диагностики возбудителей описторхоза и меторхоза» 2018-2020 гг



«PCR-test for detection and differentiation of opisthorchiasis and metorchosis pathogens» 2018-2020.


Введение серологического исследования на эболавирус для тестирования клинических случаев с негативными результатами ПЦР-тестов может также изменить окончательное число лабораторно подтвержденных случаев заболевания.



The recent introduction of ebolavirus serology to test PCR negative clinical cases is also likely to change the final number of laboratory confirmed cases.


аллергопробы, онкомаркеры, ПЦР-тесты на инфекции и другие специфические анализы



allergy tests, tumor markers, PCR tests for infections and other specific tests,


Проблема заключается в том, что большинство ПЦР-тестов длится минимум час, а окно для принятия врачом решения обычно не превышает десяти минут.



The problem is that most PCR tests can take up to an hour or more, and a physician’s decision-making window is typically less than 10 minutes.


Возможно получение результатов ПЦР-теста в течение одного дня.


Молекулярный ответ может быть определен, когда ПЦР-тест используется для нахождения гена слияния BCR-ABL.



A molecular response is when the PCR test is used to find the BCR-ABL fusion gene.


Но обычные ПЦР-тесты могут определять около 20 похожих вирусов за раз, в то время как тест-система ViroCap теоретически способна определить любой вирус.



However, while PCR tests can screen for up to about 20 similar viruses at one time, it seems that ViroCap could theoretically test for virtually any virus.


Но обычные ПЦР-тесты могут определять около 20 похожих вирусов за раз, в то время как тест-система ViroCap теоретически способна определить любой вирус.



But even PCR tests are only capable of screening for up to 20 viruses at a time; ViroCap could hypothetically test for essentially any virus.

Ничего не найдено для этого значения.

Результатов: 179. Точных совпадений: 2. Затраченное время: 85 мс

Documents

Корпоративные решения

Спряжение

Синонимы

Корректор

Справка и о нас

Индекс слова: 1-300, 301-600, 601-900

Индекс выражения: 1-400, 401-800, 801-1200

Индекс фразы: 1-400, 401-800, 801-1200

Ты вовсе не центр вселенной!

последовательность полиморфических локусов, прогоняем через расширенный процесс тестирования с использованием ПЦР

Это очень интересно.

Not everything’s beneath you!

And then we extract sequenced polymorphic loci from the patient, put it through an amplified testing process using PCR.

Very exciting.

Можешь провести

ПЦР этих образцов, глянуть, есть ли генетические маркеры?

— Думаешь… дефицит ингибитора трипсина?

Ha! Ha!

Um, can you run a PCR on these samples, see if there are any — genetic markers? — You’re thinking…

Antitrypsin deficiency?

Результат биопсии очень непонятный, соответственно, мы провели цитологию, и снова ничего.

— тест на ПЦР?

И опять ничего.

Biopsy came back unreadable, so we ran cytology: nothing.

PCR?

Still nothing.

— Извините.

Мне пришлось поработать над ПЦР.

— Ага, и собака съела мою домашнюю работу.

— Sorry.

I had to babysit a PCR assay.

— Yeah, and the dog ate my homework.

Ну хорошо, тогда закончили.

Вернёмся к ПЦР диагностике.

Хорошо.

Okay, I think we’re done.

Let’s get to the PCRs.

Okay.

Либо мы найдем лежащее в основе этого заболевание, либо он солгал своему последнему избирателю.

Ложный положительный ПЦР тест на СПИД?

Сделал его дважды.

Unless we figure out the underlying disease, this guy’s lied to his last constituent.

— False negative on the P.C.R. AIDS test?

— Ran it twice.

Это редкость, но любой из вирусов гепатита может вызывать нарушения сна и печёночную недостаточность.

Нет, ПЦР в норме. — У нас есть этическая дилемма.

— Нет, у нас её нет.

It’s rare,but any of the hepatitis viruses could cause sleep disturbances and liver failure.

No,pcrs were normal.—We have an ethical dilemma.

No,we don’T.

С другой стороны, у меня, есть вот это.

Ты провёл на мне анализ ПЦР, без моего согласия?

Эй, это хорошие новости. Правда?

I, on the other hand, have this.

You ran a PCR on me without my consent?

— Hey, it’s good news.

Ладно, Кемп, у нас есть образцы ДНК предполагаемых отцов, расположенные по дате.

Центрифуга, ПЦР, секвенсер.

Думаю, вы знакомы с процессом?

OK, Kemp, so we have the contested father DNA samples, all in date order.

Erm, centrifuge, PCR, sequencer.

I take it you’re familiar with the process?

Что ты делаешь?

Анализ ПЦР.

Ты делаешь его лично? Среди ночи.

— What are you doing?

— PCR test.

You’re doing it yourself in the middle of the night.

Если не можешь защитить — не считается

Забронируй процедурную. проведите эндоскопию верхней части тела и ПЦР тест на болезнь Уиппла.

Виноват/а

Counts for nothing if you can’t defend ii.

Book a procedure room, do an upper endoscopy, and run a PCR test for Whipple’s.

Guilt.

Эндоскопия не выявила никаких нарушений В тонком кишечнике

Я сейчас провожу анализ ПЦР.

Нашли это в моем кабинете.

Endoscopy didn’t reveal any lesions in the small intestine.

I’m running the PCR test now.

I found it in my office.

Да?

Считыватель имунно-ферментного анализа, амплификатор для ПЦР,

— клеточный инкубатор.

Yeah?

ELISA plate reader, PCR thermocyclers,

— cell culture incubator.

Я провел тесты, чтобы убедиться в том, что он не заразен.

Но вирус успел мутировать в той части последовательности, которую не смогла обнаружить ПЦР.

По крайне мере, теперь я смогу изолировать антитела и, возможно…

The tests I ran to make sure he wasn’t contagious.

The virus happened to mutate exactly within the target sequence of my PCR primers, which produced a false negative.

At least now I can isolate his antibodies and perhaps…

Идем.

Нужно сделать ПЦР, используя больше ваших клеток.

Потому что моя ДНК другая.

Let’s go.

We need to do the PCR using a larger extract of your cells.

Because my DNA is different.

Использовать инопланетную ДНК, чтобы победить Спартанский вирус, создав вакцину против него.

Вы проведёте ПЦР, используя образцы моего генома.

Хорошо, на это не уйдёт много времени.

Using alien DNA to defeat the Spartan Virus by creating a vaccine against it.

You’re gonna do a PCR, using primers from my sequence.

Okay, that shouldn’t take long.

Натали?

Сделай количественную ПЦР некоторых пятен.

А потом капиллярный электрофорез.

Natalie. I need you to do

A quantitative PCR on some fluid stains.

And then a capillary electrophoresis. We’d like you to cross-check it

На этой неделе мы должны предоставить сведения о распределении обязанностей.

Барри, возьми результаты ПЦР.

Карл, выясни…

We need to hand out attorney assignments for the week.

Barry, I need you to take the PCR testing.

Carl, discovery…

Синегнойка.

ПЦР выше в два раза.

Я могу попытаться стабилизировать может, через подключичную вену.

Pseudomonas.

PCR positive times two.

I can go in, try to get local control, maybe subclavian repair.

Судя по её странице на фейсбуке, она — первая скрипка в Королевском театре.

Ну что ж, Рейнальдо проведет ПЦР-амплификацию найденного образца ДНК.

Так мы узнаем пол и расу убийцы.

According to her Facebook page she’s a first chair violinist in a production at the Kings Theater.

All right, I have got Reynaldo going back through the PCR amplification of the touched DNA.

Could give us race and gender.

Термостабильная ДНК-полимераза.

Выявляется ПЦР в течение 4 часов.

Подготовишь фолликул?

Taq polymerase.

This is going into PCR for four hours.

Can you prep the eyebrow follicle?

Показать еще

1) исследование; испытание; тест, проба; опыт; анализ

2) исследовать; испытывать; проверять; производить анализ

3) реакция

antibody adsorption test — тест адсорбции антител

autourine test — проба с внутрикожным введением аутологичной мочи, аутоуротест

Bachman’s test — проба Бахмана

band test — бэнд-тест

bed-side test — 1) анализ у постели больного, прикроватная проба 2) карточная проба Элдона

Biken test — проба Бикена

blood-bank test — скрининг донорского банка крови; массовое типирование групп крови

Calmett’s test — тест [проба] Кальметта

cis-trans test — тест на цис-транс положение, цис-транс тест

Coombs’ test — реакция [проба] Кумбса, антиглобулиновый тест

C1q deviation test — тест остаточного C1q-субкомпонента комплемента

cross-immunization test — проба с родственным антигеном, проба на перекрёстную иммунологическую специфичность

crude test — грубая [предварительная] проба

Ducrey’s test — проба Дюкре

enzyme-Coombs’ test — антиглобулиновый тест с ферментативным усилением, иммуноферментная проба Кумбса

erythrocyte adherence test — реакция иммуноагглютинации эритроцитов, реакция иммунного прилипания эритроцитов

Field-Caspari test — тест для определения электрофоретической подвижности макрофагов, проба Фильда-Каспари

fragility test — проба на резистентность эритроцитов, тест для определения осмотической резистентности эритроцитов, эритроцит-резистентный тест

Franci’s test — кожная проба Франци

FTA-ABS test — тест с антитрепонемальными флуоресцирующими антителами

Geller’s test — реакция Геллера

Gruber’s test — реакция Грубера

Heller’s test — дифференциальный тест агглютинации, тест Хеллера

Jensen’s test — проба Йенсена, внутрикожная проба Йенсена на кроликах

Kahn’s test — реакция Кана, осадочная [цитохолевая] реакция Кана

Kleinhauer’s test — проба Клейнхауера

latex allergosorbent test — латексный аллергосорбентный тест, аллергосорбентный тест на частицах латекса

Lee-Davidsohn test — проба [тест] Ли-Дэвидсона

loose-body test — тест высвобождения волчаночных клеток, LEC-рилизинг тест

beta-lysin disk test — дисковая проба с beta-лизином, beta-лизиновый диск-тест

mitogen-stimulation test — проба со стимуляцией митогеном, реакция митогензависимой пролиферации ; реакция смешанной культуры лимфоцитов, СКЛ-реакция

MLC test — реакция смешанной культуры лимфоцитов, СКЛ-реакция

Oehlecker’s test — биологическая проба на совместимость, проба Элеккера

oral provocation test — пероральная проба с аллергеном, провокационная проба с пероральным приёмом аллергена

PCR test — тест с полимеразным усилением

phagocytosis inhibition test — тест торможения [подавления] фагоцитоза

photopatch test — кожный тест на фоточувствительность, фотоаппликационная проба

plaque-reduction test — реакция подавления бляшкообразования, реакция подавления гемолиза

PCR test — тест с полимеразным усилением

red cell adherence test — реакция иммуноагглютинации эритроцитов, реакция иммунного прилипания эритроцитов

ring test — реакция кольцепреципитации, кольцепреципитиновый тест

rough test — грубый [предварительный] тест

routine test — стандартный [конвенциональный] тест

screening test — проба при массовом обследования; скрининг-тест

secondary stimulation test — тест вторичной стимуляции в смешанной культуре лимфоцитов, реакция во вторичной смешанной культуре лимфоцитов

sedimentation test — проба для определения скорости осаждения эритроцитов, СОЭ-тест

serum test — серологическая реакция, серотест; сывороточная проба

serum bactericidal test — проба на бактерицидные свойства сыворотки, сывороточный бактерицидный тест

Shelly’s test — тест [реакция] дегрануляции базофилов, тест [реакция] Шелли

Simonsen’s test — проба Симонсена

SPACE test — твердофазный иммуноанализ с использованием иммобилизованных комплексов антитело-эритроцит

Stormont’s test — двухэтапная внутрикожная проба с туберкулином, проба Стормонта

streptex test — латекс-агглютинационный тест на стрептококк, стрептекс-тест

superoxide scavenger activity test — тест на утилизацию супероксидных радикалов, супероксид-утилизационный тест, супероксид-дисмутазный тест

supplement test — добавочный тест, сапплемент-тест

tanned red cell test — метод пассивной агглютинации, метод Бойдена, тест таннизированных эритроцитов

VDRL test — тест Научно-исследовательской лаборатории по изучению венерических заболеваний, VDRL-тест

vitality test — определение [проверка] жизнеспособности

Welch’s test — проба Велша

A strip of eight PCR tubes, each containing a 100 μL reaction mixture

The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA,[1] were jointly awarded the Nobel Prize in Chemistry in 1993.

PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and criminal forensics.[2][3]

The majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions—specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents—primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.

Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was heat-susceptible, it would denature under the high temperatures of the denaturation step. Before the use of Taq polymerase, DNA polymerase had to be manually added every cycle, which was a tedious and costly process.[4]

Applications of the technique include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis; construction of DNA-based phylogenies, or functional analysis of genes; diagnosis and monitoring of genetic disorders; amplification of ancient DNA;[5] analysis of genetic fingerprints for DNA profiling (for example, in forensic science and parentage testing); and detection of pathogens in nucleic acid tests for the diagnosis of infectious diseases.

Principles[edit]

PCR amplifies a specific region of a DNA strand (the DNA target). Most PCR methods amplify DNA fragments of between 0.1 and 10 kilo base pairs (kbp) in length, although some techniques allow for amplification of fragments up to 40 kbp.[6] The amount of amplified product is determined by the available substrates in the reaction, which becomes limiting as the reaction progresses.[7]

A basic PCR set-up requires several components and reagents,[8] including:

  • a DNA template that contains the DNA target region to amplify
  • a DNA polymerase; an enzyme that polymerizes new DNA strands; heat-resistant Taq polymerase is especially common,[9] as it is more likely to remain intact during the high-temperature DNA denaturation process
  • two DNA primers that are complementary to the 3′ (three prime) ends of each of the sense and anti-sense strands of the DNA target (DNA polymerase can only bind to and elongate from a double-stranded region of DNA; without primers, there is no double-stranded initiation site at which the polymerase can bind);[10] specific primers that are complementary to the DNA target region are selected beforehand, and are often custom-made in a laboratory or purchased from commercial biochemical suppliers
  • deoxynucleoside triphosphates, or dNTPs (sometimes called «deoxynucleotide triphosphates»; nucleotides containing triphosphate groups), the building blocks from which the DNA polymerase synthesizes a new DNA strand
  • a buffer solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase
  • bivalent cations, typically magnesium (Mg) or manganese (Mn) ions; Mg2+ is the most common, but Mn2+ can be used for PCR-mediated DNA mutagenesis, as a higher Mn2+ concentration increases the error rate during DNA synthesis;[11] and monovalent cations, typically potassium (K) ions[better source needed]

The reaction is commonly carried out in a volume of 10–200 μL in small reaction tubes (0.2–0.5 mL volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction (see below). Many modern thermal cyclers make use of the Peltier effect, which permits both heating and cooling of the block holding the PCR tubes simply by reversing the electric current. Thin-walled reaction tubes permit favorable thermal conductivity to allow for rapid thermal equilibrium. Most thermal cyclers have heated lids to prevent condensation at the top of the reaction tube. Older thermal cyclers lacking a heated lid require a layer of oil on top of the reaction mixture or a ball of wax inside the tube.

Procedure[edit]

Typically, PCR consists of a series of 20–40 repeated temperature changes, called thermal cycles, with each cycle commonly consisting of two or three discrete temperature steps (see figure below). The cycling is often preceded by a single temperature step at a very high temperature (>90 °C (194 °F)), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters, including the enzyme used for DNA synthesis, the concentration of bivalent ions and dNTPs in the reaction, and the melting temperature (Tm) of the primers.[12] The individual steps common to most PCR methods are as follows:

  • Initialization: This step is only required for DNA polymerases that require heat activation by hot-start PCR.[13] It consists of heating the reaction chamber to a temperature of 94–96 °C (201–205 °F), or 98 °C (208 °F) if extremely thermostable polymerases are used, which is then held for 1–10 minutes.
  • Denaturation: This step is the first regular cycling event and consists of heating the reaction chamber to 94–98 °C (201–208 °F) for 20–30 seconds. This causes DNA melting, or denaturation, of the double-stranded DNA template by breaking the hydrogen bonds between complementary bases, yielding two single-stranded DNA molecules.
  • Annealing: In the next step, the reaction temperature is lowered to 50–65 °C (122–149 °F) for 20–40 seconds, allowing annealing of the primers to each of the single-stranded DNA templates. Two different primers are typically included in the reaction mixture: one for each of the two single-stranded complements containing the target region. The primers are single-stranded sequences themselves, but are much shorter than the length of the target region, complementing only very short sequences at the 3′ end of each strand.
It is critical to determine a proper temperature for the annealing step because efficiency and specificity are strongly affected by the annealing temperature. This temperature must be low enough to allow for hybridization of the primer to the strand, but high enough for the hybridization to be specific, i.e., the primer should bind only to a perfectly complementary part of the strand, and nowhere else. If the temperature is too low, the primer may bind imperfectly. If it is too high, the primer may not bind at all. A typical annealing temperature is about 3–5 °C below the Tm of the primers used. Stable hydrogen bonds between complementary bases are formed only when the primer sequence very closely matches the template sequence. During this step, the polymerase binds to the primer-template hybrid and begins DNA formation.
  • Extension/elongation: The temperature at this step depends on the DNA polymerase used; the optimum activity temperature for the thermostable DNA polymerase of Taq polymerase is approximately 75–80 °C (167–176 °F),[14][15] though a temperature of 72 °C (162 °F) is commonly used with this enzyme. In this step, the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding free dNTPs from the reaction mixture that is complementary to the template in the 5′-to-3′ direction, condensing the 5′-phosphate group of the dNTPs with the 3′-hydroxy group at the end of the nascent (elongating) DNA strand. The precise time required for elongation depends both on the DNA polymerase used and on the length of the DNA target region to amplify. As a rule of thumb, at their optimal temperature, most DNA polymerases polymerize a thousand bases per minute. Under optimal conditions (i.e., if there are no limitations due to limiting substrates or reagents), at each extension/elongation step, the number of DNA target sequences is doubled. With each successive cycle, the original template strands plus all newly generated strands become template strands for the next round of elongation, leading to exponential (geometric) amplification of the specific DNA target region.
The processes of denaturation, annealing and elongation constitute a single cycle. Multiple cycles are required to amplify the DNA target to millions of copies. The formula used to calculate the number of DNA copies formed after a given number of cycles is 2n, where n is the number of cycles. Thus, a reaction set for 30 cycles results in 230, or 1,073,741,824, copies of the original double-stranded DNA target region.
  • Final elongation: This single step is optional, but is performed at a temperature of 70–74 °C (158–165 °F) (the temperature range required for optimal activity of most polymerases used in PCR) for 5–15 minutes after the last PCR cycle to ensure that any remaining single-stranded DNA is fully elongated.
  • Final hold: The final step cools the reaction chamber to 4–15 °C (39–59 °F) for an indefinite time, and may be employed for short-term storage of the PCR products.

Schematic drawing of a complete PCR cycle

Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue samples. No amplification is present in sample #1; DNA bands in sample #2 and #3 indicate successful amplification of the target sequence. The gel also shows a positive control, and a DNA ladder containing DNA fragments of defined length for sizing the bands in the experimental PCRs.

To check whether the PCR successfully generated the anticipated DNA target region (also sometimes referred to as the amplimer or amplicon), agarose gel electrophoresis may be employed for size separation of the PCR products. The size of the PCR products is determined by comparison with a DNA ladder, a molecular weight marker which contains DNA fragments of known sizes, which runs on the gel alongside the PCR products.

Tucker PCR

Stages[edit]

Exponential amplification

As with other chemical reactions, the reaction rate and efficiency of PCR are affected by limiting factors. Thus, the entire PCR process can further be divided into three stages based on reaction progress:

  • Exponential amplification: At every cycle, the amount of product is doubled (assuming 100% reaction efficiency). After 30 cycles, a single copy of DNA can be increased up to 1,000,000,000 (one billion) copies. In a sense, then, the replication of a discrete strand of DNA is being manipulated in a tube under controlled conditions.[16] The reaction is very sensitive: only minute quantities of DNA must be present.
  • Leveling off stage: The reaction slows as the DNA polymerase loses activity and as consumption of reagents, such as dNTPs and primers, causes them to become more limited.
  • Plateau: No more product accumulates due to exhaustion of reagents and enzyme.

Optimization[edit]

In practice, PCR can fail for various reasons, such as sensitivity or contamination.[17][18] Contamination with extraneous DNA can lead to spurious products and is addressed with lab protocols and procedures that separate pre-PCR mixtures from potential DNA contaminants.[8] For instance, if DNA from a crime scene is analyzed, a single DNA molecule from lab personnel could be amplified and misguide the investigation. Hence the PCR-setup areas is separated from the analysis or purification of other PCR products, disposable plasticware used, and the work surface between reaction setups needs to be thoroughly cleaned.

Specificity can be adjusted by experimental conditions so that no spurious products are generated. Primer-design techniques are important in improving PCR product yield and in avoiding the formation of unspecific products. The usage of alternate buffer components or polymerase enzymes can help with amplification of long or otherwise problematic regions of DNA. For instance, Q5 polymerase is said to be ~280 times less error-prone than Taq polymerase.[19][20] Both the running parameters (e.g. temperature and duration of cycles), or the addition of reagents, such as formamide, may increase the specificity and yield of PCR.[21] Computer simulations of theoretical PCR results (Electronic PCR) may be performed to assist in primer design.[22]

Applications[edit]

Selective DNA isolation[edit]

PCR allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. This use of PCR augments many ways, such as generating hybridization probes for Southern or northern hybridization and DNA cloning, which require larger amounts of DNA, representing a specific DNA region. PCR supplies these techniques with high amounts of pure DNA, enabling analysis of DNA samples even from very small amounts of starting material.

Other applications of PCR include DNA sequencing to determine unknown PCR-amplified sequences in which one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid, phage, or cosmid (depending on size) or the genetic material of another organism. Bacterial colonies (such as E. coli) can be rapidly screened by PCR for correct DNA vector constructs.[23] PCR may also be used for genetic fingerprinting; a forensic technique used to identify a person or organism by comparing experimental DNAs through different PCR-based methods.

Electrophoresis of PCR-amplified DNA fragments:

  1. Father
  2. Child
  3. Mother

The child has inherited some, but not all, of the fingerprints of each of its parents, giving it a new, unique fingerprint.

Some PCR fingerprint methods have high discriminative power and can be used to identify genetic relationships between individuals, such as parent-child or between siblings, and are used in paternity testing (Fig. 4). This technique may also be used to determine evolutionary relationships among organisms when certain molecular clocks are used (i.e. the 16S rRNA and recA genes of microorganisms).[24]

Amplification and quantification of DNA[edit]

Because PCR amplifies the regions of DNA that it targets, PCR can be used to analyze extremely small amounts of sample. This is often critical for forensic analysis, when only a trace amount of DNA is available as evidence. PCR may also be used in the analysis of ancient DNA that is tens of thousands of years old. These PCR-based techniques have been successfully used on animals, such as a forty-thousand-year-old mammoth, and also on human DNA, in applications ranging from the analysis of Egyptian mummies to the identification of a Russian tsar and the body of English king Richard III.[25]

Quantitative PCR or Real Time PCR (qPCR,[26] not to be confused with RT-PCR) methods allow the estimation of the amount of a given sequence present in a sample—a technique often applied to quantitatively determine levels of gene expression. Quantitative PCR is an established tool for DNA quantification that measures the accumulation of DNA product after each round of PCR amplification.

qPCR allows the quantification and detection of a specific DNA sequence in real time since it measures concentration while the synthesis process is taking place. There are two methods for simultaneous detection and quantification. The first method consists of using fluorescent dyes that are retained nonspecifically in between the double strands. The second method involves probes that code for specific sequences and are fluorescently labeled. Detection of DNA using these methods can only be seen after the hybridization of probes with its complementary DNA (cDNA) takes place. An interesting technique combination is real-time PCR and reverse transcription. This sophisticated technique, called RT-qPCR, allows for the quantification of a small quantity of RNA. Through this combined technique, mRNA is converted to cDNA, which is further quantified using qPCR. This technique lowers the possibility of error at the end point of PCR,[27] increasing chances for detection of genes associated with genetic diseases such as cancer.[5] Laboratories use RT-qPCR for the purpose of sensitively measuring gene regulation. The mathematical foundations for the reliable quantification of the PCR[28] and RT-qPCR[29] facilitate the implementation of accurate fitting procedures of experimental data in research, medical, diagnostic and infectious disease applications.[30][31][32][33]

Medical and diagnostic applications[edit]

Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease.[2] DNA samples for prenatal testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother’s bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.

  • PCR can also be used as part of a sensitive test for tissue typing, vital to organ transplantation. As of 2008, there is even a proposal to replace the traditional antibody-based tests for blood type with PCR-based tests.[34]
  • Many forms of cancer involve alterations to oncogenes. By using PCR-based tests to study these mutations, therapy regimens can sometimes be individually customized to a patient. PCR permits early diagnosis of malignant diseases such as leukemia and lymphomas, which is currently the highest-developed in cancer research and is already being used routinely. PCR assays can be performed directly on genomic DNA samples to detect translocation-specific malignant cells at a sensitivity that is at least 10,000 fold higher than that of other methods.[35] PCR is very useful in the medical field since it allows for the isolation and amplification of tumor suppressors. Quantitative PCR for example, can be used to quantify and analyze single cells, as well as recognize DNA, mRNA and protein confirmations and combinations.[27]

Infectious disease applications[edit]

PCR allows for rapid and highly specific diagnosis of infectious diseases, including those caused by bacteria or viruses.[36] PCR also permits identification of non-cultivatable or slow-growing microorganisms such as mycobacteria, anaerobic bacteria, or viruses from tissue culture assays and animal models. The basis for PCR diagnostic applications in microbiology is the detection of infectious agents and the discrimination of non-pathogenic from pathogenic strains by virtue of specific genes.[36][37]

Characterization and detection of infectious disease organisms have been revolutionized by PCR in the following ways:

  • The human immunodeficiency virus (or HIV), is a difficult target to find and eradicate. The earliest tests for infection relied on the presence of antibodies to the virus circulating in the bloodstream. However, antibodies don’t appear until many weeks after infection, maternal antibodies mask the infection of a newborn, and therapeutic agents to fight the infection don’t affect the antibodies. PCR tests have been developed that can detect as little as one viral genome among the DNA of over 50,000 host cells.[38] Infections can be detected earlier, donated blood can be screened directly for the virus, newborns can be immediately tested for infection, and the effects of antiviral treatments can be quantified.
  • Some disease organisms, such as that for tuberculosis, are difficult to sample from patients and slow to be grown in the laboratory. PCR-based tests have allowed detection of small numbers of disease organisms (both live or dead), in convenient samples. Detailed genetic analysis can also be used to detect antibiotic resistance, allowing immediate and effective therapy. The effects of therapy can also be immediately evaluated.
  • The spread of a disease organism through populations of domestic or wild animals can be monitored by PCR testing. In many cases, the appearance of new virulent sub-types can be detected and monitored. The sub-types of an organism that were responsible for earlier epidemics can also be determined by PCR analysis.
  • Viral DNA can be detected by PCR. The primers used must be specific to the targeted sequences in the DNA of a virus, and PCR can be used for diagnostic analyses or DNA sequencing of the viral genome. The high sensitivity of PCR permits virus detection soon after infection and even before the onset of disease.[36] Such early detection may give physicians a significant lead time in treatment. The amount of virus («viral load») in a patient can also be quantified by PCR-based DNA quantitation techniques (see below). A variant of PCR (RT-PCR) is used for detecting viral RNA rather than DNA: in this test the enzyme reverse transcriptase is used to generate a DNA sequence which matches the viral RNA; this DNA is then amplified as per the usual PCR method. RT-PCR is widely used to detect the SARS-CoV-2 viral genome.[39]
  • Diseases such as pertussis (or whooping cough) are caused by the bacteria Bordetella pertussis. This bacteria is marked by a serious acute respiratory infection that affects various animals and humans and has led to the deaths of many young children. The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such as T lymphocytes which play a role in cell immunity.[40] PCR is an important testing tool that can detect sequences within the gene for the pertussis toxin. Because PCR has a high sensitivity for the toxin and a rapid turnaround time, it is very efficient for diagnosing pertussis when compared to culture.[41]

Forensic applications[edit]

The development of PCR-based genetic (or DNA) fingerprinting protocols has seen widespread application in forensics:

  • DNA samples are often taken at crime scenes and analyzed by PCR.

    In its most discriminating form, genetic fingerprinting can uniquely discriminate any one person from the entire population of the world. Minute samples of DNA can be isolated from a crime scene, and compared to that from suspects, or from a DNA database of earlier evidence or convicts. Simpler versions of these tests are often used to rapidly rule out suspects during a criminal investigation. Evidence from decades-old crimes can be tested, confirming or exonerating the people originally convicted.

  • Forensic DNA typing has been an effective way of identifying or exonerating criminal suspects due to analysis of evidence discovered at a crime scene. The human genome has many repetitive regions that can be found within gene sequences or in non-coding regions of the genome. Specifically, up to 40% of human DNA is repetitive.[5] There are two distinct categories for these repetitive, non-coding regions in the genome. The first category is called variable number tandem repeats (VNTR), which are 10–100 base pairs long and the second category is called short tandem repeats (STR) and these consist of repeated 2–10 base pair sections. PCR is used to amplify several well-known VNTRs and STRs using primers that flank each of the repetitive regions. The sizes of the fragments obtained from any individual for each of the STRs will indicate which alleles are present. By analyzing several STRs for an individual, a set of alleles for each person will be found that statistically is likely to be unique.[5] Researchers have identified the complete sequence of the human genome. This sequence can be easily accessed through the NCBI website and is used in many real-life applications. For example, the FBI has compiled a set of DNA marker sites used for identification, and these are called the Combined DNA Index System (CODIS) DNA database.[5] Using this database enables statistical analysis to be used to determine the probability that a DNA sample will match. PCR is a very powerful and significant analytical tool to use for forensic DNA typing because researchers only need a very small amount of the target DNA to be used for analysis. For example, a single human hair with attached hair follicle has enough DNA to conduct the analysis. Similarly, a few sperm, skin samples from under the fingernails, or a small amount of blood can provide enough DNA for conclusive analysis.[5]
  • Less discriminating forms of DNA fingerprinting can help in DNA paternity testing, where an individual is matched with their close relatives. DNA from unidentified human remains can be tested, and compared with that from possible parents, siblings, or children. Similar testing can be used to confirm the biological parents of an adopted (or kidnapped) child. The actual biological father of a newborn can also be confirmed (or ruled out).
  • The PCR AMGX/AMGY design has been shown to not only[clarification needed] facilitate in amplifying DNA sequences from a very minuscule amount of genome. However it can also be used for real-time sex determination from forensic bone samples. This provides a powerful and effective way to determine gender in forensic cases and ancient specimens.[42]

Research applications[edit]

PCR has been applied to many areas of research in molecular genetics:

  • PCR allows rapid production of short pieces of DNA, even when not more than the sequence of the two primers is known. This ability of PCR augments many methods, such as generating hybridization probes for Southern or northern blot hybridization. PCR supplies these techniques with large amounts of pure DNA, sometimes as a single strand, enabling analysis even from very small amounts of starting material.
  • The task of DNA sequencing can also be assisted by PCR. Known segments of DNA can easily be produced from a patient with a genetic disease mutation. Modifications to the amplification technique can extract segments from a completely unknown genome, or can generate just a single strand of an area of interest.
  • PCR has numerous applications to the more traditional process of DNA cloning. It can extract segments for insertion into a vector from a larger genome, which may be only available in small quantities. Using a single set of ‘vector primers’, it can also analyze or extract fragments that have already been inserted into vectors. Some alterations to the PCR protocol can generate mutations (general or site-directed) of an inserted fragment.
  • Sequence-tagged sites is a process where PCR is used as an indicator that a particular segment of a genome is present in a particular clone. The Human Genome Project found this application vital to mapping the cosmid clones they were sequencing, and to coordinating the results from different laboratories.
  • An application of PCR is the phylogenic analysis of DNA from ancient sources, such as that found in the recovered bones of Neanderthals, from frozen tissues of mammoths, or from the brain of Egyptian mummies.[16] In some cases the highly degraded DNA from these sources might be reassembled during the early stages of amplification.
  • A common application of PCR is the study of patterns of gene expression. Tissues (or even individual cells) can be analyzed at different stages to see which genes have become active, or which have been switched off. This application can also use quantitative PCR to quantitate the actual levels of expression
  • The ability of PCR to simultaneously amplify several loci from individual sperm[43] has greatly enhanced the more traditional task of genetic mapping by studying chromosomal crossovers after meiosis. Rare crossover events between very close loci have been directly observed by analyzing thousands of individual sperms. Similarly, unusual deletions, insertions, translocations, or inversions can be analyzed, all without having to wait (or pay) for the long and laborious processes of fertilization, embryogenesis, etc.
  • Site-directed mutagenesis: PCR can be used to create mutant genes with mutations chosen by scientists at will. These mutations can be chosen in order to understand how proteins accomplish their functions, and to change or improve protein function.

Advantages[edit]

PCR has a number of advantages. It is fairly simple to understand and to use, and produces results rapidly. The technique is highly sensitive with the potential to produce millions to billions of copies of a specific product for sequencing, cloning, and analysis. qRT-PCR shares the same advantages as the PCR, with an added advantage of quantification of the synthesized product. Therefore, it has its uses to analyze alterations of gene expression levels in tumors, microbes, or other disease states.[27]

PCR is a very powerful and practical research tool. The sequencing of unknown etiologies of many diseases are being figured out by the PCR. The technique can help identify the sequence of previously unknown viruses related to those already known and thus give us a better understanding of the disease itself. If the procedure can be further simplified and sensitive non-radiometric detection systems can be developed, the PCR will assume a prominent place in the clinical laboratory for years to come.[16]

Limitations[edit]

One major limitation of PCR is that prior information about the target sequence is necessary in order to generate the primers that will allow its selective amplification.[27] This means that, typically, PCR users must know the precise sequence(s) upstream of the target region on each of the two single-stranded templates in order to ensure that the DNA polymerase properly binds to the primer-template hybrids and subsequently generates the entire target region during DNA synthesis.

Like all enzymes, DNA polymerases are also prone to error, which in turn causes mutations in the PCR fragments that are generated.[44]

Another limitation of PCR is that even the smallest amount of contaminating DNA can be amplified, resulting in misleading or ambiguous results. To minimize the chance of contamination, investigators should reserve separate rooms for reagent preparation, the PCR, and analysis of product. Reagents should be dispensed into single-use aliquots. Pipettors with disposable plungers and extra-long pipette tips should be routinely used.[16] It is moreover recommended to ensure that the lab set-up follows a unidirectional workflow. No materials or reagents used in the PCR and analysis rooms should ever be taken into the PCR preparation room without thorough decontamination.[45]

Environmental samples that contain humic acids may inhibit PCR amplification and lead to inaccurate results.

Variations[edit]

  • Allele-specific PCR: a diagnostic or cloning technique based on single-nucleotide variations (SNVs not to be confused with SNPs) (single-base differences in a patient). It requires prior knowledge of a DNA sequence, including differences between alleles, and uses primers whose 3′ ends encompass the SNV (base pair buffer around SNV usually incorporated). PCR amplification under stringent conditions is much less efficient in the presence of a mismatch between template and primer, so successful amplification with an SNP-specific primer signals presence of the specific SNP in a sequence.[46] See SNP genotyping for more information.
  • Assembly PCR or Polymerase Cycling Assembly (PCA): artificial synthesis of long DNA sequences by performing PCR on a pool of long oligonucleotides with short overlapping segments. The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments, thereby selectively producing the final long DNA product.[47]
  • Asymmetric PCR: preferentially amplifies one DNA strand in a double-stranded DNA template. It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required. PCR is carried out as usual, but with a great excess of the primer for the strand targeted for amplification. Because of the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required.[48] A recent modification on this process, known as Linear-After-The-Exponential-PCR (LATE-PCR), uses a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.[49]
  • Convective PCR: a pseudo-isothermal way of performing PCR. Instead of repeatedly heating and cooling the PCR mixture, the solution is subjected to a thermal gradient. The resulting thermal instability driven convective flow automatically shuffles the PCR reagents from the hot and cold regions repeatedly enabling PCR.[50] Parameters such as thermal boundary conditions and geometry of the PCR enclosure can be optimized to yield robust and rapid PCR by harnessing the emergence of chaotic flow fields.[51] Such convective flow PCR setup significantly reduces device power requirement and operation time.
  • Dial-out PCR: a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR.[52]
  • Digital PCR (dPCR): used to measure the quantity of a target DNA sequence in a DNA sample. The DNA sample is highly diluted so that after running many PCRs in parallel, some of them do not receive a single molecule of the target DNA. The target DNA concentration is calculated using the proportion of negative outcomes. Hence the name ‘digital PCR’.
  • Helicase-dependent amplification: similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension cycles. DNA helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation.[53]
  • Hot start PCR: a technique that reduces non-specific amplification during the initial set up stages of the PCR. It may be performed manually by heating the reaction components to the denaturation temperature (e.g., 95 °C) before adding the polymerase.[54] Specialized enzyme systems have been developed that inhibit the polymerase’s activity at ambient temperature, either by the binding of an antibody[13][55] or by the presence of covalently bound inhibitors that dissociate only after a high-temperature activation step. Hot-start/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are instantly activated at elongation temperature.
  • In silico PCR (digital PCR, virtual PCR, electronic PCR, e-PCR) refers to computational tools used to calculate theoretical polymerase chain reaction results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome. In silico PCR was proposed as an educational tool for molecular biology.[56]
  • Intersequence-specific PCR (ISSR): a PCR method for DNA fingerprinting that amplifies regions between simple sequence repeats to produce a unique fingerprint of amplified fragment lengths.[57]
  • Inverse PCR: is commonly used to identify the flanking sequences around genomic inserts. It involves a series of DNA digestions and self ligation, resulting in known sequences at either end of the unknown sequence.[58]
  • Ligation-mediated PCR: uses small DNA linkers ligated to the DNA of interest and multiple primers annealing to the DNA linkers; it has been used for DNA sequencing, genome walking, and DNA footprinting.[59]
  • Methylation-specific PCR (MSP): developed by Stephen Baylin and James G. Herman at the Johns Hopkins School of Medicine,[60] and is used to detect methylation of CpG islands in genomic DNA. DNA is first treated with sodium bisulfite, which converts unmethylated cytosine bases to uracil, which is recognized by PCR primers as thymine. Two PCRs are then carried out on the modified DNA, using primer sets identical except at any CpG islands within the primer sequences. At these points, one primer set recognizes DNA with cytosines to amplify methylated DNA, and one set recognizes DNA with uracil or thymine to amplify unmethylated DNA. MSP using qPCR can also be performed to obtain quantitative rather than qualitative information about methylation.
  • Miniprimer PCR: uses a thermostable polymerase (S-Tbr) that can extend from short primers («smalligos») as short as 9 or 10 nucleotides. This method permits PCR targeting to smaller primer binding regions, and is used to amplify conserved DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene.[61]
  • Multiplex ligation-dependent probe amplification (MLPA): permits amplifying multiple targets with a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below).
  • Multiplex-PCR: consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes. That is, their base pair length should be different enough to form distinct bands when visualized by gel electrophoresis.
  • Nanoparticle-assisted PCR (nanoPCR): some nanoparticles (NPs) can enhance the efficiency of PCR (thus being called nanoPCR), and some can even outperform the original PCR enhancers. It was reported that quantum dots (QDs) can improve PCR specificity and efficiency. Single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) are efficient in enhancing the amplification of long PCR. Carbon nanopowder (CNP) can improve the efficiency of repeated PCR and long PCR, while zinc oxide, titanium dioxide and Ag NPs were found to increase the PCR yield. Previous data indicated that non-metallic NPs retained acceptable amplification fidelity. Given that many NPs are capable of enhancing PCR efficiency, it is clear that there is likely to be great potential for nanoPCR technology improvements and product development.[62][63]
  • Nested PCR: increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. Two sets of primers are used in two successive PCRs. In the first reaction, one pair of primers is used to generate DNA products, which besides the intended target, may still consist of non-specifically amplified DNA fragments. The product(s) are then used in a second PCR with a set of primers whose binding sites are completely or partially different from and located 3′ of each of the primers used in the first reaction. Nested PCR is often more successful in specifically amplifying long DNA fragments than conventional PCR, but it requires more detailed knowledge of the target sequences.
  • Overlap-extension PCR or Splicing by overlap extension (SOEing) : a genetic engineering technique that is used to splice together two or more DNA fragments that contain complementary sequences. It is used to join DNA pieces containing genes, regulatory sequences, or mutations; the technique enables creation of specific and long DNA constructs. It can also introduce deletions, insertions or point mutations into a DNA sequence.[64][65]
  • PAN-AC: uses isothermal conditions for amplification, and may be used in living cells.[66][67]
  • Quantitative PCR (qPCR): used to measure the quantity of a target sequence (commonly in real-time). It quantitatively measures starting amounts of DNA, cDNA, or RNA. Quantitative PCR is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample. Quantitative PCR has a very high degree of precision. Quantitative PCR methods use fluorescent dyes, such as Sybr Green, EvaGreen or fluorophore-containing DNA probes, such as TaqMan, to measure the amount of amplified product in real time. It is also sometimes abbreviated to RT-PCR (real-time PCR) but this abbreviation should be used only for reverse transcription PCR. qPCR is the appropriate contractions for quantitative PCR (real-time PCR).
  • Reverse Complement PCR (RC-PCR): Allows the addition of functional domains or sequences of choice to be appended independently to either end of the generated amplicon in a single closed tube reaction. This method generates target specific primers within the reaction by the interaction of universal primers (which contain the desired sequences or domains to be appended) and RC probes.
  • Reverse Transcription PCR (RT-PCR): for amplifying DNA from RNA. Reverse transcriptase reverse transcribes RNA into cDNA, which is then amplified by PCR. RT-PCR is widely used in expression profiling, to determine the expression of a gene or to identify the sequence of an RNA transcript, including transcription start and termination sites. If the genomic DNA sequence of a gene is known, RT-PCR can be used to map the location of exons and introns in the gene. The 5′ end of a gene (corresponding to the transcription start site) is typically identified by RACE-PCR (Rapid Amplification of cDNA Ends).
  • RNase H-dependent PCR (rhPCR): a modification of PCR that utilizes primers with a 3′ extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for multiplexed reactions to be performed with higher numbers of primers.[68]
  • Single specific primer-PCR (SSP-PCR): allows the amplification of double-stranded DNA even when the sequence information is available at one end only. This method permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome.[69]
  • Solid Phase PCR: encompasses multiple meanings, including Polony Amplification (where PCR colonies are derived in a gel matrix, for example), Bridge PCR[70] (primers are covalently linked to a solid-support surface), conventional Solid Phase PCR (where Asymmetric PCR is applied in the presence of solid support bearing primer with sequence matching one of the aqueous primers) and Enhanced Solid Phase PCR[71] (where conventional Solid Phase PCR can be improved by employing high Tm and nested solid support primer with optional application of a thermal ‘step’ to favour solid support priming).
  • Suicide PCR: typically used in paleogenetics or other studies where avoiding false positives and ensuring the specificity of the amplified fragment is the highest priority. It was originally described in a study to verify the presence of the microbe Yersinia pestis in dental samples obtained from 14th Century graves of people supposedly killed by the plague during the medieval Black Death epidemic.[72] The method prescribes the use of any primer combination only once in a PCR (hence the term «suicide»), which should never have been used in any positive control PCR reaction, and the primers should always target a genomic region never amplified before in the lab using this or any other set of primers. This ensures that no contaminating DNA from previous PCR reactions is present in the lab, which could otherwise generate false positives.
  • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence.[73]
  • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at the initial cycles is usually a few degrees (3–5 °C) above the Tm of the primers used, while at the later cycles, it is a few degrees (3–5 °C) below the primer Tm. The higher temperatures give greater specificity for primer binding, and the lower temperatures permit more efficient amplification from the specific products formed during the initial cycles.[74]
  • Universal Fast Walking: for genome walking and genetic fingerprinting using a more specific ‘two-sided’ PCR than conventional ‘one-sided’ approaches (using only one gene-specific primer and one general primer—which can lead to artefactual ‘noise’)[75] by virtue of a mechanism involving lariat structure formation. Streamlined derivatives of UFW are LaNe RAGE (lariat-dependent nested PCR for rapid amplification of genomic DNA ends),[76] 5’RACE LaNe[77] and 3’RACE LaNe.[78]

History[edit]

Diagrammatic representation of an example primer pair. The use of primers in an in vitro assay to allow DNA synthesis was a major innovation that allowed the development of PCR.

The heat-resistant enzymes that are a key component in polymerase chain reaction were discovered in the 1960s as a product of a microbial life form that lived in the superheated waters of Yellowstone’s Mushroom Spring.[79]

A 1971 paper in the Journal of Molecular Biology by Kjell Kleppe and co-workers in the laboratory of H. Gobind Khorana first described a method of using an enzymatic assay to replicate a short DNA template with primers in vitro.[80] However, this early manifestation of the basic PCR principle did not receive much attention at the time and the invention of the polymerase chain reaction in 1983 is generally credited to Kary Mullis.[81][page needed]

«Baby Blue», a 1986 prototype machine for doing PCR

When Mullis developed the PCR in 1983, he was working in Emeryville, California for Cetus Corporation, one of the first biotechnology companies, where he was responsible for synthesizing short chains of DNA. Mullis has written that he conceived the idea for PCR while cruising along the Pacific Coast Highway one night in his car.[82] He was playing in his mind with a new way of analyzing changes (mutations) in DNA when he realized that he had instead invented a method of amplifying any DNA region through repeated cycles of duplication driven by DNA polymerase. In Scientific American, Mullis summarized the procedure: «Beginning with a single molecule of the genetic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. The reaction is easy to execute. It requires no more than a test tube, a few simple reagents, and a source of heat.»[83] DNA fingerprinting was first used for paternity testing in 1988.[84]

Mullis has credited his use of LSD as integral to his development of PCR: «Would I have invented PCR if I hadn’t taken LSD? I seriously doubt it. I could sit on a DNA molecule and watch the polymers go by. I learnt that partly on psychedelic drugs.»[85]

Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA,[1] were jointly awarded the Nobel Prize in Chemistry in 1993, seven years after Mullis and his colleagues at Cetus first put his proposal to practice.[86] Mullis’s 1985 paper with R. K. Saiki and H. A. Erlich, «Enzymatic Amplification of β-globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia»—the polymerase chain reaction invention (PCR)—was honored by a Citation for Chemical Breakthrough Award from the Division of History of Chemistry of the American Chemical Society in 2017.[87][2]

At the core of the PCR method is the use of a suitable DNA polymerase able to withstand the high temperatures of >90 °C (194 °F) required for separation of the two DNA strands in the DNA double helix after each replication cycle. The DNA polymerases initially employed for in vitro experiments presaging PCR were unable to withstand these high temperatures.[2] So the early procedures for DNA replication were very inefficient and time-consuming, and required large amounts of DNA polymerase and continuous handling throughout the process.

The discovery in 1976 of Taq polymerase—a DNA polymerase purified from the thermophilic bacterium, Thermus aquaticus, which naturally lives in hot (50 to 80 °C (122 to 176 °F)) environments[14] such as hot springs—paved the way for dramatic improvements of the PCR method. The DNA polymerase isolated from T. aquaticus is stable at high temperatures remaining active even after DNA denaturation,[15] thus obviating the need to add new DNA polymerase after each cycle.[3] This allowed an automated thermocycler-based process for DNA amplification.

Patent disputes[edit]

The PCR technique was patented by Kary Mullis and assigned to Cetus Corporation, where Mullis worked when he invented the technique in 1983. The Taq polymerase enzyme was also covered by patents. There have been several high-profile lawsuits related to the technique, including an unsuccessful lawsuit brought by DuPont. The Swiss pharmaceutical company Hoffmann-La Roche purchased the rights to the patents in 1992. The last of the commercial PCR patents expired in 2017.[88]

A related patent battle over the Taq polymerase enzyme is still ongoing[as of?] in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on 28 March 2005.[89]

See also[edit]

  • COVID-19 testing
  • DNA spiking
  • Loop-mediated isothermal amplification
  • Selector technique
  • Thermus thermophilus
  • Pfu DNA polymerase

References[edit]

  1. ^ a b «The Nobel Prize in Chemistry 1993». NobelPrize.org.
  2. ^ a b c d Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (December 1985). «Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia». Science. 230 (4732): 1350–54. Bibcode:1985Sci…230.1350S. doi:10.1126/science.2999980. PMID 2999980.
  3. ^ a b Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. (January 1988). «Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase». Science. 239 (4839): 487–91. Bibcode:1988Sci…239..487S. doi:10.1126/science.239.4839.487. PMID 2448875.
  4. ^ Enners, Edward; Porta, Angela R. (2012). «Determining Annealing Temperatures for Polymerase Chain Reaction». The American Biology Teacher. 74 (4): 256–60. doi:10.1525/abt.2012.74.4.9. S2CID 86708426.
  5. ^ a b c d e f Ninfa, Alexander; Ballou, David; Benore, Marilee (2009). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. United States: Wiley. pp. 408–10. ISBN 978-0-470-08766-4.
  6. ^ Cheng S, Fockler C, Barnes WM, Higuchi R (June 1994). «Effective amplification of long targets from cloned inserts and human genomic DNA». Proceedings of the National Academy of Sciences of the United States of America. 91 (12): 5695–99. Bibcode:1994PNAS…91.5695C. doi:10.1073/pnas.91.12.5695. PMC 44063. PMID 8202550.
  7. ^ Carr AC, Moore SD (2012). Lucia A (ed.). «Robust quantification of polymerase chain reactions using global fitting». PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO…737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
  8. ^ a b Joseph Sambrook & David W. Russel (2001). Molecular Cloning: A Laboratory Manual (third ed.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. ISBN 978-0-879-69576-7. Chapter 8: In vitro Amplification of DNA by the Polymerase Chain Reaction
  9. ^ «Polymerase Chain Reaction (PCR)». National Center for Biotechnology Information, U.S. National Library of Medicine.
  10. ^ «PCR». Genetic Science Learning Center, University of Utah.
  11. ^ Pavlov AR, Pavlova NV, Kozyavkin SA, Slesarev AI (May 2004). «Recent developments in the optimization of thermostable DNA polymerases for efficient applications». Trends in Biotechnology. 22 (5): 253–60. doi:10.1016/j.tibtech.2004.02.011. PMID 15109812.
  12. ^ Rychlik W, Spencer WJ, Rhoads RE (November 1990). «Optimization of the annealing temperature for DNA amplification in vitro». Nucleic Acids Research. 18 (21): 6409–12. doi:10.1093/nar/18.21.6409. PMC 332522. PMID 2243783.
  13. ^ a b Sharkey DJ, Scalice ER, Christy KG, Atwood SM, Daiss JL (May 1994). «Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction». Bio/Technology. 12 (5): 506–09. doi:10.1038/nbt0594-506. PMID 7764710. S2CID 2885453.
  14. ^ a b Chien A, Edgar DB, Trela JM (September 1976). «Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus». Journal of Bacteriology. 127 (3): 1550–57. doi:10.1128/jb.127.3.1550-1557.1976. PMC 232952. PMID 8432.
  15. ^ a b Lawyer FC, Stoffel S, Saiki RK, Chang SY, Landre PA, Abramson RD, Gelfand DH (May 1993). «High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity». PCR Methods and Applications. 2 (4): 275–87. doi:10.1101/gr.2.4.275. PMID 8324500.
  16. ^ a b c d Schochetman G, Ou CY, Jones WK (December 1988). «Polymerase chain reaction». The Journal of Infectious Diseases. 158 (6): 1154–57. doi:10.1093/infdis/158.6.1154. JSTOR 30137034. PMID 2461996.
  17. ^ Borman, Jon; Schuster, David; Li, Wu-bo; Jessee, Joel; Rashtchian, Ayoub (2000). «PCR from problematic templates» (PDF). Focus. 22 (1): 10. Archived from the original (PDF) on 7 March 2017.
  18. ^ Bogetto, Prachi; Waidne, Lisa; Anderson, Holly (2000). «Helpful tips for PCR» (PDF). Focus. 22 (1): 12. Archived from the original (PDF) on 7 March 2017.
  19. ^ Biolabs, New England. «Q5® High-Fidelity DNA Polymerase | NEB». www.neb.com. Retrieved 4 December 2021.
  20. ^ Sze, Marc A.; Schloss, Patrick D. (2019). «The Impact of DNA Polymerase and Number of Rounds of Amplification in PCR on 16S rRNA Gene Sequence Data». mSphere. 4 (3): e00163–19. doi:10.1128/mSphere.00163-19. PMC 6531881. PMID 31118299.
  21. ^ Sarkar G, Kapelner S, Sommer SS (December 1990). «Formamide can dramatically improve the specificity of PCR». Nucleic Acids Research. 18 (24): 7465. doi:10.1093/nar/18.24.7465. PMC 332902. PMID 2259646.
  22. ^ «Electronic PCR». NCBI – National Center for Biotechnology Information. Retrieved 13 March 2012.
  23. ^ Pavlov AR, Pavlova NV, Kozyavkin SA, Slesarev AI (2006). «Thermostable DNA Polymerases for a Wide Spectrum of Applications: Comparison of a Robust Hybrid TopoTaq to other enzymes». In Kieleczawa J (ed.). DNA Sequencing II: Optimizing Preparation and Cleanup. Jones & Bartlett. pp. 241–57. ISBN 978-0-7637-3383-4.
  24. ^ Pombert JF, Sistek V, Boissinot M, Frenette M (October 2009). «Evolutionary relationships among salivarius streptococci as inferred from multilocus phylogenies based on 16S rRNA-encoding, recA, secA, and secY gene sequences». BMC Microbiology. 9: 232. doi:10.1186/1471-2180-9-232. PMC 2777182. PMID 19878555.
  25. ^ «Chemical Synthesis, Sequencing, and Amplification of DNA (class notes on MBB/BIO 343)». Arizona State University. Archived from the original on 9 October 1997. Retrieved 29 October 2007.
  26. ^ Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. (April 2009). «The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments» (PDF). Clinical Chemistry. 55 (4): 611–22. doi:10.1373/clinchem.2008.112797. PMID 19246619.
  27. ^ a b c d Garibyan L, Avashia N (March 2013). «Polymerase chain reaction». The Journal of Investigative Dermatology. 133 (3): 1–4. doi:10.1038/jid.2013.1. PMC 4102308. PMID 23399825.
  28. ^ Schnell, S.; Mendoza, C. (October 1997). «Theoretical Description of the Polymerase Chain Reaction». Journal of Theoretical Biology. 188 (3): 313–18. Bibcode:1997JThBi.188..313S. doi:10.1006/jtbi.1997.0473. PMID 9344735.
  29. ^ Schnell, S.; Mendoza, C. (21 February 1997). «Enzymological Considerations for the Theoretical Description of the Quantitative Competitive Polymerase Chain Reaction (QC-PCR)». Journal of Theoretical Biology. 184 (4): 433–40. Bibcode:1997JThBi.184..433S. doi:10.1006/jtbi.1996.0283. ISSN 0022-5193. PMID 9082073.
  30. ^ Becker, Sven; Böger, Peter; Oehlmann, Ralfh; Ernst, Anneliese (1 November 2000). «PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities». Applied and Environmental Microbiology. 66 (11): 4945–53. Bibcode:2000ApEnM..66.4945B. doi:10.1128/AEM.66.11.4945-4953.2000. ISSN 1098-5336. PMC 92404. PMID 11055948.
  31. ^ Solomon, Anthony W.; Peeling, Rosanna W.; Foster, Allen; Mabey, David C. W. (1 October 2004). «Diagnosis and Assessment of Trachoma». Clinical Microbiology Reviews. 17 (4): 982–1011. doi:10.1128/CMR.17.4.982-1011.2004. ISSN 0893-8512. PMC 523557. PMID 15489358.
  32. ^ Ramzy, Reda M.R. (April 2002). «Recent advances in molecular diagnostic techniques for human lymphatic filariasis and their use in epidemiological research». Transactions of the Royal Society of Tropical Medicine and Hygiene. 96: S225–29. doi:10.1016/S0035-9203(02)90080-5. PMID 12055843.
  33. ^ Sachse, Konrad (2003). «Specificity and performance of diagnostic PCR assays». In Sachse, Konrad; Frey, Joachim (eds.). PCR Detection of Microbial Pathogens. Methods in Molecular Biology. Vol. 216. Totowa, New Jersey: Humana Press. pp. 3–29. doi:10.1385/1-59259-344-5:03. ISBN 978-1-59259-344-6. PMID 12512353.
  34. ^ Quill E (March 2008). «Medicine. Blood-matching goes genetic». Science. 319 (5869): 1478–79. doi:10.1126/science.319.5869.1478. PMID 18339916. S2CID 36945291.
  35. ^ Tomar, Rukam (2010). Molecular Markers and Plant Biotechnology. Pitman Pura, New Delhi: New India Publishing Agency. p. 188. ISBN 978-93-80235-25-7.
  36. ^ a b c Cai HY, Caswell JL, Prescott JF (March 2014). «Nonculture molecular techniques for diagnosis of bacterial disease in animals: a diagnostic laboratory perspective». Veterinary Pathology. 51 (2): 341–50. doi:10.1177/0300985813511132. PMID 24569613.
  37. ^ Salis AD (2009). «Applications in Clinical Microbiology». Real-Time PCR: Current Technology and Applications. Caister Academic Press. ISBN 978-1-904455-39-4.
  38. ^ Kwok S, Mack DH, Mullis KB, Poiesz B, Ehrlich G, Blair D, et al. (May 1987). «Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection». Journal of Virology. 61 (5): 1690–94. doi:10.1128/jvi.61.5.1690-1694.1987. PMC 254157. PMID 2437321.
  39. ^ «Coronavirus: il viaggio dei test». Istituto Superiore di Sanità.
  40. ^ Finger, Horst; von Koenig, Carl Heinz Wirsing (1996). Baron, Samuel (ed.). Medical Microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch at Galveston. ISBN 978-0-9631172-1-2. PMID 21413270.
  41. ^ Yeh, Sylvia H.; Mink, ChrisAnna M. (2012). «Bordetella pertussis and Pertussis (Whooping Cough)». Netter’s Infectious Diseases. Netter’s Infectious Diseases. pp. 11–14. doi:10.1016/B978-1-4377-0126-5.00003-3. ISBN 978-1-4377-0126-5.
  42. ^ Alonso A, Martín P, Albarrán C, García P, García O, de Simón LF, et al. (January 2004). «Real-Time PCR Designs to Estimate Nuclear and Mitochondrial DNA Copy Number in Forensic and Ancient DNA Studies». Forensic Science International. 139 (2–3): 141–49. doi:10.1016/j.forsciint.2003.10.008. PMID 15040907.
  43. ^ Boehnke M, Arnheim N, Li H, Collins FS (July 1989). «Fine-structure genetic mapping of human chromosomes using the polymerase chain reaction on single sperm: experimental design considerations». American Journal of Human Genetics. 45 (1): 21–32. PMC 1683385. PMID 2568090.
  44. ^ Zhou YH, Zhang XP, Ebright RH (November 1991). «Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase». Nucleic Acids Research. 19 (21): 6052. doi:10.1093/nar/19.21.6052. PMC 329070. PMID 1658751.
  45. ^ Stursberg, Stephanie (2021). «Setting up a PCR lab from scratch». INTEGRA Biosciences.{{cite web}}: CS1 maint: url-status (link)
  46. ^ Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, et al. (April 1989). «Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)». Nucleic Acids Research. 17 (7): 2503–16. doi:10.1093/nar/17.7.2503. PMC 317639. PMID 2785681.
  47. ^ Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (October 1995). «Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides». Gene. 164 (1): 49–53. doi:10.1016/0378-1119(95)00511-4. PMID 7590320.
  48. ^ Innis MA, Myambo KB, Gelfand DH, Brow MA (December 1988). «DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA». Proceedings of the National Academy of Sciences of the United States of America. 85 (24): 9436–40. Bibcode:1988PNAS…85.9436I. doi:10.1073/pnas.85.24.9436. PMC 282767. PMID 3200828.
  49. ^ Pierce KE, Wangh LJ (2007). Linear-after-the-exponential polymerase chain reaction and allied technologies Real-time detection strategies for rapid, reliable diagnosis from single cells. Methods in Molecular Medicine. Vol. 132. pp. 65–85. doi:10.1007/978-1-59745-298-4_7. ISBN 978-1-58829-578-1. PMID 17876077.
  50. ^ Krishnan M, Ugaz VM, Burns MA (October 2002). «PCR in a Rayleigh-Bénard convection cell». Science. 298 (5594): 793. doi:10.1126/science.298.5594.793. PMID 12399582.
  51. ^ Priye A, Hassan YA, Ugaz VM (November 2013). «Microscale chaotic advection enables robust convective DNA replication». Analytical Chemistry. 85 (21): 10536–41. doi:10.1021/ac402611s. PMID 24083802.
  52. ^ Schwartz JJ, Lee C, Shendure J (September 2012). «Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules». Nature Methods. 9 (9): 913–15. doi:10.1038/nmeth.2137. PMC 3433648. PMID 22886093.
  53. ^ Vincent M, Xu Y, Kong H (August 2004). «Helicase-dependent isothermal DNA amplification». EMBO Reports. 5 (8): 795–800. doi:10.1038/sj.embor.7400200. PMC 1249482. PMID 15247927.
  54. ^ Chou Q, Russell M, Birch DE, Raymond J, Bloch W (April 1992). «Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications». Nucleic Acids Research. 20 (7): 1717–23. doi:10.1093/nar/20.7.1717. PMC 312262. PMID 1579465.
  55. ^ Kellogg DE, Rybalkin I, Chen S, Mukhamedova N, Vlasik T, Siebert PD, Chenchik A (June 1994). «TaqStart Antibody: «hot start» PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase». BioTechniques. 16 (6): 1134–37. PMID 8074881.
  56. ^ San Millán RM, Martínez-Ballesteros I, Rementeria A, Garaizar J, Bikandi J (December 2013). «Online exercise for the design and simulation of PCR and PCR-RFLP experiments». BMC Research Notes. 6: 513. doi:10.1186/1756-0500-6-513. PMC 4029544. PMID 24314313.
  57. ^ Zietkiewicz E, Rafalski A, Labuda D (March 1994). «Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification». Genomics. 20 (2): 176–83. doi:10.1006/geno.1994.1151. PMID 8020964.
  58. ^ Ochman H, Gerber AS, Hartl DL (November 1988). «Genetic applications of an inverse polymerase chain reaction». Genetics. 120 (3): 621–23. doi:10.1093/genetics/120.3.621. PMC 1203539. PMID 2852134.
  59. ^ Mueller PR, Wold B (November 1989). «In vivo footprinting of a muscle specific enhancer by ligation mediated PCR». Science. 246 (4931): 780–86. Bibcode:1989Sci…246..780M. doi:10.1126/science.2814500. PMID 2814500.
  60. ^ Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB (September 1996). «Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands». Proceedings of the National Academy of Sciences of the United States of America. 93 (18): 9821–26. Bibcode:1996PNAS…93.9821H. doi:10.1073/pnas.93.18.9821. PMC 38513. PMID 8790415.
  61. ^ Isenbarger TA, Finney M, Ríos-Velázquez C, Handelsman J, Ruvkun G (February 2008). «Miniprimer PCR, a new lens for viewing the microbial world». Applied and Environmental Microbiology. 74 (3): 840–49. Bibcode:2008ApEnM..74..840I. doi:10.1128/AEM.01933-07. PMC 2227730. PMID 18083877.
  62. ^ Shen C, Yang W, Ji Q, Maki H, Dong A, Zhang Z (November 2009). «NanoPCR observation: different levels of DNA replication fidelity in nanoparticle-enhanced polymerase chain reactions». Nanotechnology. 20 (45): 455103. Bibcode:2009Nanot..20S5103S. doi:10.1088/0957-4484/20/45/455103. PMID 19822925. S2CID 3393115.
  63. ^ Shen, Cenchao (2013). «An Overview of Nanoparticle-Assisted Polymerase Chain Reaction Technology». An Overview of Nanoparticle‐Assisted Polymerase Chain Reaction Technology. US: Wiley-Blackwell Publishing Ltd. pp. 97–106. doi:10.1002/9781118451915.ch5. ISBN 978-1-118-45191-5.
  64. ^ Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (April 1989). «Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension». Gene. 77 (1): 61–68. doi:10.1016/0378-1119(89)90359-4. PMID 2744488.
  65. ^ Moller, Simon (2006). PCR: The Basics. US: Taylor & Francis Group. p. 144. ISBN 978-0-415-35547-6.
  66. ^ David F, Turlotte E (November 1998). «[A method of isothermal gene amplification]» [An Isothermal Amplification Method]. Comptes Rendus de l’Académie des Sciences, Série III. 321 (11): 909–14. Bibcode:1998CRASG.321..909D. doi:10.1016/S0764-4469(99)80005-5. PMID 9879470.
  67. ^ Fabrice David (September–October 2002). «Utiliser les propriétés topologiques de l’ADN: une nouvelle arme contre les agents pathogènes» (PDF). Fusion. Archived from the original (PDF) on 28 November 2007.(in French)
  68. ^ Dobosy JR, Rose SD, Beltz KR, Rupp SM, Powers KM, Behlke MA, Walder JA (August 2011). «RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers». BMC Biotechnology. 11: 80. doi:10.1186/1472-6750-11-80. PMC 3224242. PMID 21831278.
  69. ^ Shyamala, V.; Ferro-Luzzi, Ames G. (1993). Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. Methods in Molecular Biology. Vol. 15. pp. 339–48. doi:10.1385/0-89603-244-2:339. ISBN 978-0-89603-244-6. PMID 21400290.
  70. ^ Bing DH, Boles C, Rehman FN, Audeh M, Belmarsh M, Kelley B, Adams CP (1996). «Bridge amplification: a solid phase PCR system for the amplification and detection of allelic differences in single copy genes». Genetic Identity Conference Proceedings, Seventh International Symposium on Human Identification. Archived from the original on 7 May 2001.
  71. ^ Khan Z, Poetter K, Park DJ (April 2008). «Enhanced solid phase PCR: mechanisms to increase priming by solid support primers». Analytical Biochemistry. 375 (2): 391–93. doi:10.1016/j.ab.2008.01.021. PMID 18267099.
  72. ^ Raoult D, Aboudharam G, Crubézy E, Larrouy G, Ludes B, Drancourt M (November 2000). «Molecular identification by «suicide PCR» of Yersinia pestis as the agent of medieval black death». Proceedings of the National Academy of Sciences of the United States of America. 97 (23): 12800–03. Bibcode:2000PNAS…9712800R. doi:10.1073/pnas.220225197. PMC 18844. PMID 11058154.
  73. ^ Liu YG, Whittier RF (February 1995). «Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking». Genomics. 25 (3): 674–81. doi:10.1016/0888-7543(95)80010-J. PMID 7759102.
  74. ^ Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (July 1991). «‘Touchdown’ PCR to circumvent spurious priming during gene amplification». Nucleic Acids Research. 19 (14): 4008. doi:10.1093/nar/19.14.4008. PMC 328507. PMID 1861999.
  75. ^ Myrick KV, Gelbart WM (February 2002). «Universal Fast Walking for direct and versatile determination of flanking sequence». Gene. 284 (1–2): 125–31. doi:10.1016/S0378-1119(02)00384-0. PMID 11891053.
  76. ^ «Full Text – LaNe RAGE: a new tool for genomic DNA flanking sequence determination». www.ejbiotechnology.info.
  77. ^ Park DJ (January 2005). «A new 5′ terminal murine GAPDH exon identified using 5’RACE LaNe». Molecular Biotechnology. 29 (1): 39–46. doi:10.1385/MB:29:1:39. PMID 15668518. S2CID 45702164.
  78. ^ Park DJ (April 2004). «3′ RACE LaNe: a simple and rapid fully nested PCR method to determine 3′-terminal cDNA sequence». BioTechniques. 36 (4): 586–88, 590. doi:10.2144/04364BM04. PMID 15088375.
  79. ^ «Key ingredient in coronavirus tests comes from Yellowstone’s lakes». Science. 31 March 2020. Retrieved 13 May 2020.
  80. ^ Kleppe K, Ohtsuka E, Kleppe R, Molineux I, Khorana HG (March 1971). «Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA’s as catalyzed by DNA polymerases». Journal of Molecular Biology. 56 (2): 341–61. doi:10.1016/0022-2836(71)90469-4. PMID 4927950.
  81. ^ Rabinow, Paul (1996). Making PCR: A Story of Biotechnology. Chicago: University of Chicago Press. ISBN 978-0-226-70146-2.
  82. ^ Mullis, Kary (1998). Dancing Naked in the Mind Field. New York: Pantheon Books. ISBN 978-0-679-44255-4.
  83. ^ Mullis KB (April 1990). «The unusual origin of the polymerase chain reaction». Scientific American. 262 (4): 56–61, 64–65. Bibcode:1990SciAm.262d..56M. doi:10.1038/scientificamerican0490-56. PMID 2315679.
  84. ^ Patidar M, Agrawal S, Parveen F, Khare P (2015). «Molecular insights of saliva in solving paternity dispute». Journal of Forensic Dental Sciences. 7 (1): 76–79. doi:10.4103/0975-1475.150325. PMC 4330625. PMID 25709326.
  85. ^ Nichols D, Barker E (2016). «Psychedelics». Pharmacological Reviews. 68 (2): 264–355. doi:10.1124/pr.115.011478. PMC 4813425. PMID 26841800.
  86. ^ «The Nobel Prize in Chemistry 1993». NobelPrize.org.
  87. ^ «Citations for Chemical Breakthrough Awards 2017 Awardees». Division of the History of Chemistry. Retrieved 12 March 2018.
  88. ^ Fore, J. Jr.; Wiechers, I. R.; Cook-Deegan, R. (3 July 2006). «The effects of business practices, licensing, and intellectual property on development and dissemination of the polymerase chain reaction: case study». Journal of Biomedical Discovery and Collaboration. 1: 7. doi:10.1186/1747-5333-1-7. PMC 1523369. PMID 16817955.
  89. ^ «Advice on How to Survive the Taq Wars». GEN Genetic Engineering News – Biobusiness Channel. 26 (9). 1 May 2006.

External links[edit]

  • US Patent for PCR
  • What is PCR plateau effect? YouTube tutorial video
  • History of the Polymerase Chain Reaction from the Smithsonian Institution Archives

A strip of eight PCR tubes, each containing a 100 μL reaction mixture

The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA,[1] were jointly awarded the Nobel Prize in Chemistry in 1993.

PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and criminal forensics.[2][3]

The majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions—specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents—primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.

Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was heat-susceptible, it would denature under the high temperatures of the denaturation step. Before the use of Taq polymerase, DNA polymerase had to be manually added every cycle, which was a tedious and costly process.[4]

Applications of the technique include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis; construction of DNA-based phylogenies, or functional analysis of genes; diagnosis and monitoring of genetic disorders; amplification of ancient DNA;[5] analysis of genetic fingerprints for DNA profiling (for example, in forensic science and parentage testing); and detection of pathogens in nucleic acid tests for the diagnosis of infectious diseases.

Principles[edit]

PCR amplifies a specific region of a DNA strand (the DNA target). Most PCR methods amplify DNA fragments of between 0.1 and 10 kilo base pairs (kbp) in length, although some techniques allow for amplification of fragments up to 40 kbp.[6] The amount of amplified product is determined by the available substrates in the reaction, which becomes limiting as the reaction progresses.[7]

A basic PCR set-up requires several components and reagents,[8] including:

  • a DNA template that contains the DNA target region to amplify
  • a DNA polymerase; an enzyme that polymerizes new DNA strands; heat-resistant Taq polymerase is especially common,[9] as it is more likely to remain intact during the high-temperature DNA denaturation process
  • two DNA primers that are complementary to the 3′ (three prime) ends of each of the sense and anti-sense strands of the DNA target (DNA polymerase can only bind to and elongate from a double-stranded region of DNA; without primers, there is no double-stranded initiation site at which the polymerase can bind);[10] specific primers that are complementary to the DNA target region are selected beforehand, and are often custom-made in a laboratory or purchased from commercial biochemical suppliers
  • deoxynucleoside triphosphates, or dNTPs (sometimes called «deoxynucleotide triphosphates»; nucleotides containing triphosphate groups), the building blocks from which the DNA polymerase synthesizes a new DNA strand
  • a buffer solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase
  • bivalent cations, typically magnesium (Mg) or manganese (Mn) ions; Mg2+ is the most common, but Mn2+ can be used for PCR-mediated DNA mutagenesis, as a higher Mn2+ concentration increases the error rate during DNA synthesis;[11] and monovalent cations, typically potassium (K) ions[better source needed]

The reaction is commonly carried out in a volume of 10–200 μL in small reaction tubes (0.2–0.5 mL volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction (see below). Many modern thermal cyclers make use of the Peltier effect, which permits both heating and cooling of the block holding the PCR tubes simply by reversing the electric current. Thin-walled reaction tubes permit favorable thermal conductivity to allow for rapid thermal equilibrium. Most thermal cyclers have heated lids to prevent condensation at the top of the reaction tube. Older thermal cyclers lacking a heated lid require a layer of oil on top of the reaction mixture or a ball of wax inside the tube.

Procedure[edit]

Typically, PCR consists of a series of 20–40 repeated temperature changes, called thermal cycles, with each cycle commonly consisting of two or three discrete temperature steps (see figure below). The cycling is often preceded by a single temperature step at a very high temperature (>90 °C (194 °F)), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters, including the enzyme used for DNA synthesis, the concentration of bivalent ions and dNTPs in the reaction, and the melting temperature (Tm) of the primers.[12] The individual steps common to most PCR methods are as follows:

  • Initialization: This step is only required for DNA polymerases that require heat activation by hot-start PCR.[13] It consists of heating the reaction chamber to a temperature of 94–96 °C (201–205 °F), or 98 °C (208 °F) if extremely thermostable polymerases are used, which is then held for 1–10 minutes.
  • Denaturation: This step is the first regular cycling event and consists of heating the reaction chamber to 94–98 °C (201–208 °F) for 20–30 seconds. This causes DNA melting, or denaturation, of the double-stranded DNA template by breaking the hydrogen bonds between complementary bases, yielding two single-stranded DNA molecules.
  • Annealing: In the next step, the reaction temperature is lowered to 50–65 °C (122–149 °F) for 20–40 seconds, allowing annealing of the primers to each of the single-stranded DNA templates. Two different primers are typically included in the reaction mixture: one for each of the two single-stranded complements containing the target region. The primers are single-stranded sequences themselves, but are much shorter than the length of the target region, complementing only very short sequences at the 3′ end of each strand.
It is critical to determine a proper temperature for the annealing step because efficiency and specificity are strongly affected by the annealing temperature. This temperature must be low enough to allow for hybridization of the primer to the strand, but high enough for the hybridization to be specific, i.e., the primer should bind only to a perfectly complementary part of the strand, and nowhere else. If the temperature is too low, the primer may bind imperfectly. If it is too high, the primer may not bind at all. A typical annealing temperature is about 3–5 °C below the Tm of the primers used. Stable hydrogen bonds between complementary bases are formed only when the primer sequence very closely matches the template sequence. During this step, the polymerase binds to the primer-template hybrid and begins DNA formation.
  • Extension/elongation: The temperature at this step depends on the DNA polymerase used; the optimum activity temperature for the thermostable DNA polymerase of Taq polymerase is approximately 75–80 °C (167–176 °F),[14][15] though a temperature of 72 °C (162 °F) is commonly used with this enzyme. In this step, the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding free dNTPs from the reaction mixture that is complementary to the template in the 5′-to-3′ direction, condensing the 5′-phosphate group of the dNTPs with the 3′-hydroxy group at the end of the nascent (elongating) DNA strand. The precise time required for elongation depends both on the DNA polymerase used and on the length of the DNA target region to amplify. As a rule of thumb, at their optimal temperature, most DNA polymerases polymerize a thousand bases per minute. Under optimal conditions (i.e., if there are no limitations due to limiting substrates or reagents), at each extension/elongation step, the number of DNA target sequences is doubled. With each successive cycle, the original template strands plus all newly generated strands become template strands for the next round of elongation, leading to exponential (geometric) amplification of the specific DNA target region.
The processes of denaturation, annealing and elongation constitute a single cycle. Multiple cycles are required to amplify the DNA target to millions of copies. The formula used to calculate the number of DNA copies formed after a given number of cycles is 2n, where n is the number of cycles. Thus, a reaction set for 30 cycles results in 230, or 1,073,741,824, copies of the original double-stranded DNA target region.
  • Final elongation: This single step is optional, but is performed at a temperature of 70–74 °C (158–165 °F) (the temperature range required for optimal activity of most polymerases used in PCR) for 5–15 minutes after the last PCR cycle to ensure that any remaining single-stranded DNA is fully elongated.
  • Final hold: The final step cools the reaction chamber to 4–15 °C (39–59 °F) for an indefinite time, and may be employed for short-term storage of the PCR products.

Schematic drawing of a complete PCR cycle

Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue samples. No amplification is present in sample #1; DNA bands in sample #2 and #3 indicate successful amplification of the target sequence. The gel also shows a positive control, and a DNA ladder containing DNA fragments of defined length for sizing the bands in the experimental PCRs.

To check whether the PCR successfully generated the anticipated DNA target region (also sometimes referred to as the amplimer or amplicon), agarose gel electrophoresis may be employed for size separation of the PCR products. The size of the PCR products is determined by comparison with a DNA ladder, a molecular weight marker which contains DNA fragments of known sizes, which runs on the gel alongside the PCR products.

Tucker PCR

Stages[edit]

Exponential amplification

As with other chemical reactions, the reaction rate and efficiency of PCR are affected by limiting factors. Thus, the entire PCR process can further be divided into three stages based on reaction progress:

  • Exponential amplification: At every cycle, the amount of product is doubled (assuming 100% reaction efficiency). After 30 cycles, a single copy of DNA can be increased up to 1,000,000,000 (one billion) copies. In a sense, then, the replication of a discrete strand of DNA is being manipulated in a tube under controlled conditions.[16] The reaction is very sensitive: only minute quantities of DNA must be present.
  • Leveling off stage: The reaction slows as the DNA polymerase loses activity and as consumption of reagents, such as dNTPs and primers, causes them to become more limited.
  • Plateau: No more product accumulates due to exhaustion of reagents and enzyme.

Optimization[edit]

In practice, PCR can fail for various reasons, such as sensitivity or contamination.[17][18] Contamination with extraneous DNA can lead to spurious products and is addressed with lab protocols and procedures that separate pre-PCR mixtures from potential DNA contaminants.[8] For instance, if DNA from a crime scene is analyzed, a single DNA molecule from lab personnel could be amplified and misguide the investigation. Hence the PCR-setup areas is separated from the analysis or purification of other PCR products, disposable plasticware used, and the work surface between reaction setups needs to be thoroughly cleaned.

Specificity can be adjusted by experimental conditions so that no spurious products are generated. Primer-design techniques are important in improving PCR product yield and in avoiding the formation of unspecific products. The usage of alternate buffer components or polymerase enzymes can help with amplification of long or otherwise problematic regions of DNA. For instance, Q5 polymerase is said to be ~280 times less error-prone than Taq polymerase.[19][20] Both the running parameters (e.g. temperature and duration of cycles), or the addition of reagents, such as formamide, may increase the specificity and yield of PCR.[21] Computer simulations of theoretical PCR results (Electronic PCR) may be performed to assist in primer design.[22]

Applications[edit]

Selective DNA isolation[edit]

PCR allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. This use of PCR augments many ways, such as generating hybridization probes for Southern or northern hybridization and DNA cloning, which require larger amounts of DNA, representing a specific DNA region. PCR supplies these techniques with high amounts of pure DNA, enabling analysis of DNA samples even from very small amounts of starting material.

Other applications of PCR include DNA sequencing to determine unknown PCR-amplified sequences in which one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid, phage, or cosmid (depending on size) or the genetic material of another organism. Bacterial colonies (such as E. coli) can be rapidly screened by PCR for correct DNA vector constructs.[23] PCR may also be used for genetic fingerprinting; a forensic technique used to identify a person or organism by comparing experimental DNAs through different PCR-based methods.

Electrophoresis of PCR-amplified DNA fragments:

  1. Father
  2. Child
  3. Mother

The child has inherited some, but not all, of the fingerprints of each of its parents, giving it a new, unique fingerprint.

Some PCR fingerprint methods have high discriminative power and can be used to identify genetic relationships between individuals, such as parent-child or between siblings, and are used in paternity testing (Fig. 4). This technique may also be used to determine evolutionary relationships among organisms when certain molecular clocks are used (i.e. the 16S rRNA and recA genes of microorganisms).[24]

Amplification and quantification of DNA[edit]

Because PCR amplifies the regions of DNA that it targets, PCR can be used to analyze extremely small amounts of sample. This is often critical for forensic analysis, when only a trace amount of DNA is available as evidence. PCR may also be used in the analysis of ancient DNA that is tens of thousands of years old. These PCR-based techniques have been successfully used on animals, such as a forty-thousand-year-old mammoth, and also on human DNA, in applications ranging from the analysis of Egyptian mummies to the identification of a Russian tsar and the body of English king Richard III.[25]

Quantitative PCR or Real Time PCR (qPCR,[26] not to be confused with RT-PCR) methods allow the estimation of the amount of a given sequence present in a sample—a technique often applied to quantitatively determine levels of gene expression. Quantitative PCR is an established tool for DNA quantification that measures the accumulation of DNA product after each round of PCR amplification.

qPCR allows the quantification and detection of a specific DNA sequence in real time since it measures concentration while the synthesis process is taking place. There are two methods for simultaneous detection and quantification. The first method consists of using fluorescent dyes that are retained nonspecifically in between the double strands. The second method involves probes that code for specific sequences and are fluorescently labeled. Detection of DNA using these methods can only be seen after the hybridization of probes with its complementary DNA (cDNA) takes place. An interesting technique combination is real-time PCR and reverse transcription. This sophisticated technique, called RT-qPCR, allows for the quantification of a small quantity of RNA. Through this combined technique, mRNA is converted to cDNA, which is further quantified using qPCR. This technique lowers the possibility of error at the end point of PCR,[27] increasing chances for detection of genes associated with genetic diseases such as cancer.[5] Laboratories use RT-qPCR for the purpose of sensitively measuring gene regulation. The mathematical foundations for the reliable quantification of the PCR[28] and RT-qPCR[29] facilitate the implementation of accurate fitting procedures of experimental data in research, medical, diagnostic and infectious disease applications.[30][31][32][33]

Medical and diagnostic applications[edit]

Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease.[2] DNA samples for prenatal testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother’s bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.

  • PCR can also be used as part of a sensitive test for tissue typing, vital to organ transplantation. As of 2008, there is even a proposal to replace the traditional antibody-based tests for blood type with PCR-based tests.[34]
  • Many forms of cancer involve alterations to oncogenes. By using PCR-based tests to study these mutations, therapy regimens can sometimes be individually customized to a patient. PCR permits early diagnosis of malignant diseases such as leukemia and lymphomas, which is currently the highest-developed in cancer research and is already being used routinely. PCR assays can be performed directly on genomic DNA samples to detect translocation-specific malignant cells at a sensitivity that is at least 10,000 fold higher than that of other methods.[35] PCR is very useful in the medical field since it allows for the isolation and amplification of tumor suppressors. Quantitative PCR for example, can be used to quantify and analyze single cells, as well as recognize DNA, mRNA and protein confirmations and combinations.[27]

Infectious disease applications[edit]

PCR allows for rapid and highly specific diagnosis of infectious diseases, including those caused by bacteria or viruses.[36] PCR also permits identification of non-cultivatable or slow-growing microorganisms such as mycobacteria, anaerobic bacteria, or viruses from tissue culture assays and animal models. The basis for PCR diagnostic applications in microbiology is the detection of infectious agents and the discrimination of non-pathogenic from pathogenic strains by virtue of specific genes.[36][37]

Characterization and detection of infectious disease organisms have been revolutionized by PCR in the following ways:

  • The human immunodeficiency virus (or HIV), is a difficult target to find and eradicate. The earliest tests for infection relied on the presence of antibodies to the virus circulating in the bloodstream. However, antibodies don’t appear until many weeks after infection, maternal antibodies mask the infection of a newborn, and therapeutic agents to fight the infection don’t affect the antibodies. PCR tests have been developed that can detect as little as one viral genome among the DNA of over 50,000 host cells.[38] Infections can be detected earlier, donated blood can be screened directly for the virus, newborns can be immediately tested for infection, and the effects of antiviral treatments can be quantified.
  • Some disease organisms, such as that for tuberculosis, are difficult to sample from patients and slow to be grown in the laboratory. PCR-based tests have allowed detection of small numbers of disease organisms (both live or dead), in convenient samples. Detailed genetic analysis can also be used to detect antibiotic resistance, allowing immediate and effective therapy. The effects of therapy can also be immediately evaluated.
  • The spread of a disease organism through populations of domestic or wild animals can be monitored by PCR testing. In many cases, the appearance of new virulent sub-types can be detected and monitored. The sub-types of an organism that were responsible for earlier epidemics can also be determined by PCR analysis.
  • Viral DNA can be detected by PCR. The primers used must be specific to the targeted sequences in the DNA of a virus, and PCR can be used for diagnostic analyses or DNA sequencing of the viral genome. The high sensitivity of PCR permits virus detection soon after infection and even before the onset of disease.[36] Such early detection may give physicians a significant lead time in treatment. The amount of virus («viral load») in a patient can also be quantified by PCR-based DNA quantitation techniques (see below). A variant of PCR (RT-PCR) is used for detecting viral RNA rather than DNA: in this test the enzyme reverse transcriptase is used to generate a DNA sequence which matches the viral RNA; this DNA is then amplified as per the usual PCR method. RT-PCR is widely used to detect the SARS-CoV-2 viral genome.[39]
  • Diseases such as pertussis (or whooping cough) are caused by the bacteria Bordetella pertussis. This bacteria is marked by a serious acute respiratory infection that affects various animals and humans and has led to the deaths of many young children. The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such as T lymphocytes which play a role in cell immunity.[40] PCR is an important testing tool that can detect sequences within the gene for the pertussis toxin. Because PCR has a high sensitivity for the toxin and a rapid turnaround time, it is very efficient for diagnosing pertussis when compared to culture.[41]

Forensic applications[edit]

The development of PCR-based genetic (or DNA) fingerprinting protocols has seen widespread application in forensics:

  • DNA samples are often taken at crime scenes and analyzed by PCR.

    In its most discriminating form, genetic fingerprinting can uniquely discriminate any one person from the entire population of the world. Minute samples of DNA can be isolated from a crime scene, and compared to that from suspects, or from a DNA database of earlier evidence or convicts. Simpler versions of these tests are often used to rapidly rule out suspects during a criminal investigation. Evidence from decades-old crimes can be tested, confirming or exonerating the people originally convicted.

  • Forensic DNA typing has been an effective way of identifying or exonerating criminal suspects due to analysis of evidence discovered at a crime scene. The human genome has many repetitive regions that can be found within gene sequences or in non-coding regions of the genome. Specifically, up to 40% of human DNA is repetitive.[5] There are two distinct categories for these repetitive, non-coding regions in the genome. The first category is called variable number tandem repeats (VNTR), which are 10–100 base pairs long and the second category is called short tandem repeats (STR) and these consist of repeated 2–10 base pair sections. PCR is used to amplify several well-known VNTRs and STRs using primers that flank each of the repetitive regions. The sizes of the fragments obtained from any individual for each of the STRs will indicate which alleles are present. By analyzing several STRs for an individual, a set of alleles for each person will be found that statistically is likely to be unique.[5] Researchers have identified the complete sequence of the human genome. This sequence can be easily accessed through the NCBI website and is used in many real-life applications. For example, the FBI has compiled a set of DNA marker sites used for identification, and these are called the Combined DNA Index System (CODIS) DNA database.[5] Using this database enables statistical analysis to be used to determine the probability that a DNA sample will match. PCR is a very powerful and significant analytical tool to use for forensic DNA typing because researchers only need a very small amount of the target DNA to be used for analysis. For example, a single human hair with attached hair follicle has enough DNA to conduct the analysis. Similarly, a few sperm, skin samples from under the fingernails, or a small amount of blood can provide enough DNA for conclusive analysis.[5]
  • Less discriminating forms of DNA fingerprinting can help in DNA paternity testing, where an individual is matched with their close relatives. DNA from unidentified human remains can be tested, and compared with that from possible parents, siblings, or children. Similar testing can be used to confirm the biological parents of an adopted (or kidnapped) child. The actual biological father of a newborn can also be confirmed (or ruled out).
  • The PCR AMGX/AMGY design has been shown to not only[clarification needed] facilitate in amplifying DNA sequences from a very minuscule amount of genome. However it can also be used for real-time sex determination from forensic bone samples. This provides a powerful and effective way to determine gender in forensic cases and ancient specimens.[42]

Research applications[edit]

PCR has been applied to many areas of research in molecular genetics:

  • PCR allows rapid production of short pieces of DNA, even when not more than the sequence of the two primers is known. This ability of PCR augments many methods, such as generating hybridization probes for Southern or northern blot hybridization. PCR supplies these techniques with large amounts of pure DNA, sometimes as a single strand, enabling analysis even from very small amounts of starting material.
  • The task of DNA sequencing can also be assisted by PCR. Known segments of DNA can easily be produced from a patient with a genetic disease mutation. Modifications to the amplification technique can extract segments from a completely unknown genome, or can generate just a single strand of an area of interest.
  • PCR has numerous applications to the more traditional process of DNA cloning. It can extract segments for insertion into a vector from a larger genome, which may be only available in small quantities. Using a single set of ‘vector primers’, it can also analyze or extract fragments that have already been inserted into vectors. Some alterations to the PCR protocol can generate mutations (general or site-directed) of an inserted fragment.
  • Sequence-tagged sites is a process where PCR is used as an indicator that a particular segment of a genome is present in a particular clone. The Human Genome Project found this application vital to mapping the cosmid clones they were sequencing, and to coordinating the results from different laboratories.
  • An application of PCR is the phylogenic analysis of DNA from ancient sources, such as that found in the recovered bones of Neanderthals, from frozen tissues of mammoths, or from the brain of Egyptian mummies.[16] In some cases the highly degraded DNA from these sources might be reassembled during the early stages of amplification.
  • A common application of PCR is the study of patterns of gene expression. Tissues (or even individual cells) can be analyzed at different stages to see which genes have become active, or which have been switched off. This application can also use quantitative PCR to quantitate the actual levels of expression
  • The ability of PCR to simultaneously amplify several loci from individual sperm[43] has greatly enhanced the more traditional task of genetic mapping by studying chromosomal crossovers after meiosis. Rare crossover events between very close loci have been directly observed by analyzing thousands of individual sperms. Similarly, unusual deletions, insertions, translocations, or inversions can be analyzed, all without having to wait (or pay) for the long and laborious processes of fertilization, embryogenesis, etc.
  • Site-directed mutagenesis: PCR can be used to create mutant genes with mutations chosen by scientists at will. These mutations can be chosen in order to understand how proteins accomplish their functions, and to change or improve protein function.

Advantages[edit]

PCR has a number of advantages. It is fairly simple to understand and to use, and produces results rapidly. The technique is highly sensitive with the potential to produce millions to billions of copies of a specific product for sequencing, cloning, and analysis. qRT-PCR shares the same advantages as the PCR, with an added advantage of quantification of the synthesized product. Therefore, it has its uses to analyze alterations of gene expression levels in tumors, microbes, or other disease states.[27]

PCR is a very powerful and practical research tool. The sequencing of unknown etiologies of many diseases are being figured out by the PCR. The technique can help identify the sequence of previously unknown viruses related to those already known and thus give us a better understanding of the disease itself. If the procedure can be further simplified and sensitive non-radiometric detection systems can be developed, the PCR will assume a prominent place in the clinical laboratory for years to come.[16]

Limitations[edit]

One major limitation of PCR is that prior information about the target sequence is necessary in order to generate the primers that will allow its selective amplification.[27] This means that, typically, PCR users must know the precise sequence(s) upstream of the target region on each of the two single-stranded templates in order to ensure that the DNA polymerase properly binds to the primer-template hybrids and subsequently generates the entire target region during DNA synthesis.

Like all enzymes, DNA polymerases are also prone to error, which in turn causes mutations in the PCR fragments that are generated.[44]

Another limitation of PCR is that even the smallest amount of contaminating DNA can be amplified, resulting in misleading or ambiguous results. To minimize the chance of contamination, investigators should reserve separate rooms for reagent preparation, the PCR, and analysis of product. Reagents should be dispensed into single-use aliquots. Pipettors with disposable plungers and extra-long pipette tips should be routinely used.[16] It is moreover recommended to ensure that the lab set-up follows a unidirectional workflow. No materials or reagents used in the PCR and analysis rooms should ever be taken into the PCR preparation room without thorough decontamination.[45]

Environmental samples that contain humic acids may inhibit PCR amplification and lead to inaccurate results.

Variations[edit]

  • Allele-specific PCR: a diagnostic or cloning technique based on single-nucleotide variations (SNVs not to be confused with SNPs) (single-base differences in a patient). It requires prior knowledge of a DNA sequence, including differences between alleles, and uses primers whose 3′ ends encompass the SNV (base pair buffer around SNV usually incorporated). PCR amplification under stringent conditions is much less efficient in the presence of a mismatch between template and primer, so successful amplification with an SNP-specific primer signals presence of the specific SNP in a sequence.[46] See SNP genotyping for more information.
  • Assembly PCR or Polymerase Cycling Assembly (PCA): artificial synthesis of long DNA sequences by performing PCR on a pool of long oligonucleotides with short overlapping segments. The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments, thereby selectively producing the final long DNA product.[47]
  • Asymmetric PCR: preferentially amplifies one DNA strand in a double-stranded DNA template. It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required. PCR is carried out as usual, but with a great excess of the primer for the strand targeted for amplification. Because of the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required.[48] A recent modification on this process, known as Linear-After-The-Exponential-PCR (LATE-PCR), uses a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.[49]
  • Convective PCR: a pseudo-isothermal way of performing PCR. Instead of repeatedly heating and cooling the PCR mixture, the solution is subjected to a thermal gradient. The resulting thermal instability driven convective flow automatically shuffles the PCR reagents from the hot and cold regions repeatedly enabling PCR.[50] Parameters such as thermal boundary conditions and geometry of the PCR enclosure can be optimized to yield robust and rapid PCR by harnessing the emergence of chaotic flow fields.[51] Such convective flow PCR setup significantly reduces device power requirement and operation time.
  • Dial-out PCR: a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR.[52]
  • Digital PCR (dPCR): used to measure the quantity of a target DNA sequence in a DNA sample. The DNA sample is highly diluted so that after running many PCRs in parallel, some of them do not receive a single molecule of the target DNA. The target DNA concentration is calculated using the proportion of negative outcomes. Hence the name ‘digital PCR’.
  • Helicase-dependent amplification: similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension cycles. DNA helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation.[53]
  • Hot start PCR: a technique that reduces non-specific amplification during the initial set up stages of the PCR. It may be performed manually by heating the reaction components to the denaturation temperature (e.g., 95 °C) before adding the polymerase.[54] Specialized enzyme systems have been developed that inhibit the polymerase’s activity at ambient temperature, either by the binding of an antibody[13][55] or by the presence of covalently bound inhibitors that dissociate only after a high-temperature activation step. Hot-start/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are instantly activated at elongation temperature.
  • In silico PCR (digital PCR, virtual PCR, electronic PCR, e-PCR) refers to computational tools used to calculate theoretical polymerase chain reaction results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome. In silico PCR was proposed as an educational tool for molecular biology.[56]
  • Intersequence-specific PCR (ISSR): a PCR method for DNA fingerprinting that amplifies regions between simple sequence repeats to produce a unique fingerprint of amplified fragment lengths.[57]
  • Inverse PCR: is commonly used to identify the flanking sequences around genomic inserts. It involves a series of DNA digestions and self ligation, resulting in known sequences at either end of the unknown sequence.[58]
  • Ligation-mediated PCR: uses small DNA linkers ligated to the DNA of interest and multiple primers annealing to the DNA linkers; it has been used for DNA sequencing, genome walking, and DNA footprinting.[59]
  • Methylation-specific PCR (MSP): developed by Stephen Baylin and James G. Herman at the Johns Hopkins School of Medicine,[60] and is used to detect methylation of CpG islands in genomic DNA. DNA is first treated with sodium bisulfite, which converts unmethylated cytosine bases to uracil, which is recognized by PCR primers as thymine. Two PCRs are then carried out on the modified DNA, using primer sets identical except at any CpG islands within the primer sequences. At these points, one primer set recognizes DNA with cytosines to amplify methylated DNA, and one set recognizes DNA with uracil or thymine to amplify unmethylated DNA. MSP using qPCR can also be performed to obtain quantitative rather than qualitative information about methylation.
  • Miniprimer PCR: uses a thermostable polymerase (S-Tbr) that can extend from short primers («smalligos») as short as 9 or 10 nucleotides. This method permits PCR targeting to smaller primer binding regions, and is used to amplify conserved DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene.[61]
  • Multiplex ligation-dependent probe amplification (MLPA): permits amplifying multiple targets with a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below).
  • Multiplex-PCR: consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes. That is, their base pair length should be different enough to form distinct bands when visualized by gel electrophoresis.
  • Nanoparticle-assisted PCR (nanoPCR): some nanoparticles (NPs) can enhance the efficiency of PCR (thus being called nanoPCR), and some can even outperform the original PCR enhancers. It was reported that quantum dots (QDs) can improve PCR specificity and efficiency. Single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) are efficient in enhancing the amplification of long PCR. Carbon nanopowder (CNP) can improve the efficiency of repeated PCR and long PCR, while zinc oxide, titanium dioxide and Ag NPs were found to increase the PCR yield. Previous data indicated that non-metallic NPs retained acceptable amplification fidelity. Given that many NPs are capable of enhancing PCR efficiency, it is clear that there is likely to be great potential for nanoPCR technology improvements and product development.[62][63]
  • Nested PCR: increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. Two sets of primers are used in two successive PCRs. In the first reaction, one pair of primers is used to generate DNA products, which besides the intended target, may still consist of non-specifically amplified DNA fragments. The product(s) are then used in a second PCR with a set of primers whose binding sites are completely or partially different from and located 3′ of each of the primers used in the first reaction. Nested PCR is often more successful in specifically amplifying long DNA fragments than conventional PCR, but it requires more detailed knowledge of the target sequences.
  • Overlap-extension PCR or Splicing by overlap extension (SOEing) : a genetic engineering technique that is used to splice together two or more DNA fragments that contain complementary sequences. It is used to join DNA pieces containing genes, regulatory sequences, or mutations; the technique enables creation of specific and long DNA constructs. It can also introduce deletions, insertions or point mutations into a DNA sequence.[64][65]
  • PAN-AC: uses isothermal conditions for amplification, and may be used in living cells.[66][67]
  • Quantitative PCR (qPCR): used to measure the quantity of a target sequence (commonly in real-time). It quantitatively measures starting amounts of DNA, cDNA, or RNA. Quantitative PCR is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample. Quantitative PCR has a very high degree of precision. Quantitative PCR methods use fluorescent dyes, such as Sybr Green, EvaGreen or fluorophore-containing DNA probes, such as TaqMan, to measure the amount of amplified product in real time. It is also sometimes abbreviated to RT-PCR (real-time PCR) but this abbreviation should be used only for reverse transcription PCR. qPCR is the appropriate contractions for quantitative PCR (real-time PCR).
  • Reverse Complement PCR (RC-PCR): Allows the addition of functional domains or sequences of choice to be appended independently to either end of the generated amplicon in a single closed tube reaction. This method generates target specific primers within the reaction by the interaction of universal primers (which contain the desired sequences or domains to be appended) and RC probes.
  • Reverse Transcription PCR (RT-PCR): for amplifying DNA from RNA. Reverse transcriptase reverse transcribes RNA into cDNA, which is then amplified by PCR. RT-PCR is widely used in expression profiling, to determine the expression of a gene or to identify the sequence of an RNA transcript, including transcription start and termination sites. If the genomic DNA sequence of a gene is known, RT-PCR can be used to map the location of exons and introns in the gene. The 5′ end of a gene (corresponding to the transcription start site) is typically identified by RACE-PCR (Rapid Amplification of cDNA Ends).
  • RNase H-dependent PCR (rhPCR): a modification of PCR that utilizes primers with a 3′ extension block that can be removed by a thermostable RNase HII enzyme. This system reduces primer-dimers and allows for multiplexed reactions to be performed with higher numbers of primers.[68]
  • Single specific primer-PCR (SSP-PCR): allows the amplification of double-stranded DNA even when the sequence information is available at one end only. This method permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome.[69]
  • Solid Phase PCR: encompasses multiple meanings, including Polony Amplification (where PCR colonies are derived in a gel matrix, for example), Bridge PCR[70] (primers are covalently linked to a solid-support surface), conventional Solid Phase PCR (where Asymmetric PCR is applied in the presence of solid support bearing primer with sequence matching one of the aqueous primers) and Enhanced Solid Phase PCR[71] (where conventional Solid Phase PCR can be improved by employing high Tm and nested solid support primer with optional application of a thermal ‘step’ to favour solid support priming).
  • Suicide PCR: typically used in paleogenetics or other studies where avoiding false positives and ensuring the specificity of the amplified fragment is the highest priority. It was originally described in a study to verify the presence of the microbe Yersinia pestis in dental samples obtained from 14th Century graves of people supposedly killed by the plague during the medieval Black Death epidemic.[72] The method prescribes the use of any primer combination only once in a PCR (hence the term «suicide»), which should never have been used in any positive control PCR reaction, and the primers should always target a genomic region never amplified before in the lab using this or any other set of primers. This ensures that no contaminating DNA from previous PCR reactions is present in the lab, which could otherwise generate false positives.
  • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence.[73]
  • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at the initial cycles is usually a few degrees (3–5 °C) above the Tm of the primers used, while at the later cycles, it is a few degrees (3–5 °C) below the primer Tm. The higher temperatures give greater specificity for primer binding, and the lower temperatures permit more efficient amplification from the specific products formed during the initial cycles.[74]
  • Universal Fast Walking: for genome walking and genetic fingerprinting using a more specific ‘two-sided’ PCR than conventional ‘one-sided’ approaches (using only one gene-specific primer and one general primer—which can lead to artefactual ‘noise’)[75] by virtue of a mechanism involving lariat structure formation. Streamlined derivatives of UFW are LaNe RAGE (lariat-dependent nested PCR for rapid amplification of genomic DNA ends),[76] 5’RACE LaNe[77] and 3’RACE LaNe.[78]

History[edit]

Diagrammatic representation of an example primer pair. The use of primers in an in vitro assay to allow DNA synthesis was a major innovation that allowed the development of PCR.

The heat-resistant enzymes that are a key component in polymerase chain reaction were discovered in the 1960s as a product of a microbial life form that lived in the superheated waters of Yellowstone’s Mushroom Spring.[79]

A 1971 paper in the Journal of Molecular Biology by Kjell Kleppe and co-workers in the laboratory of H. Gobind Khorana first described a method of using an enzymatic assay to replicate a short DNA template with primers in vitro.[80] However, this early manifestation of the basic PCR principle did not receive much attention at the time and the invention of the polymerase chain reaction in 1983 is generally credited to Kary Mullis.[81][page needed]

«Baby Blue», a 1986 prototype machine for doing PCR

When Mullis developed the PCR in 1983, he was working in Emeryville, California for Cetus Corporation, one of the first biotechnology companies, where he was responsible for synthesizing short chains of DNA. Mullis has written that he conceived the idea for PCR while cruising along the Pacific Coast Highway one night in his car.[82] He was playing in his mind with a new way of analyzing changes (mutations) in DNA when he realized that he had instead invented a method of amplifying any DNA region through repeated cycles of duplication driven by DNA polymerase. In Scientific American, Mullis summarized the procedure: «Beginning with a single molecule of the genetic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. The reaction is easy to execute. It requires no more than a test tube, a few simple reagents, and a source of heat.»[83] DNA fingerprinting was first used for paternity testing in 1988.[84]

Mullis has credited his use of LSD as integral to his development of PCR: «Would I have invented PCR if I hadn’t taken LSD? I seriously doubt it. I could sit on a DNA molecule and watch the polymers go by. I learnt that partly on psychedelic drugs.»[85]

Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA,[1] were jointly awarded the Nobel Prize in Chemistry in 1993, seven years after Mullis and his colleagues at Cetus first put his proposal to practice.[86] Mullis’s 1985 paper with R. K. Saiki and H. A. Erlich, «Enzymatic Amplification of β-globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia»—the polymerase chain reaction invention (PCR)—was honored by a Citation for Chemical Breakthrough Award from the Division of History of Chemistry of the American Chemical Society in 2017.[87][2]

At the core of the PCR method is the use of a suitable DNA polymerase able to withstand the high temperatures of >90 °C (194 °F) required for separation of the two DNA strands in the DNA double helix after each replication cycle. The DNA polymerases initially employed for in vitro experiments presaging PCR were unable to withstand these high temperatures.[2] So the early procedures for DNA replication were very inefficient and time-consuming, and required large amounts of DNA polymerase and continuous handling throughout the process.

The discovery in 1976 of Taq polymerase—a DNA polymerase purified from the thermophilic bacterium, Thermus aquaticus, which naturally lives in hot (50 to 80 °C (122 to 176 °F)) environments[14] such as hot springs—paved the way for dramatic improvements of the PCR method. The DNA polymerase isolated from T. aquaticus is stable at high temperatures remaining active even after DNA denaturation,[15] thus obviating the need to add new DNA polymerase after each cycle.[3] This allowed an automated thermocycler-based process for DNA amplification.

Patent disputes[edit]

The PCR technique was patented by Kary Mullis and assigned to Cetus Corporation, where Mullis worked when he invented the technique in 1983. The Taq polymerase enzyme was also covered by patents. There have been several high-profile lawsuits related to the technique, including an unsuccessful lawsuit brought by DuPont. The Swiss pharmaceutical company Hoffmann-La Roche purchased the rights to the patents in 1992. The last of the commercial PCR patents expired in 2017.[88]

A related patent battle over the Taq polymerase enzyme is still ongoing[as of?] in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on 28 March 2005.[89]

See also[edit]

  • COVID-19 testing
  • DNA spiking
  • Loop-mediated isothermal amplification
  • Selector technique
  • Thermus thermophilus
  • Pfu DNA polymerase

References[edit]

  1. ^ a b «The Nobel Prize in Chemistry 1993». NobelPrize.org.
  2. ^ a b c d Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (December 1985). «Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia». Science. 230 (4732): 1350–54. Bibcode:1985Sci…230.1350S. doi:10.1126/science.2999980. PMID 2999980.
  3. ^ a b Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. (January 1988). «Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase». Science. 239 (4839): 487–91. Bibcode:1988Sci…239..487S. doi:10.1126/science.239.4839.487. PMID 2448875.
  4. ^ Enners, Edward; Porta, Angela R. (2012). «Determining Annealing Temperatures for Polymerase Chain Reaction». The American Biology Teacher. 74 (4): 256–60. doi:10.1525/abt.2012.74.4.9. S2CID 86708426.
  5. ^ a b c d e f Ninfa, Alexander; Ballou, David; Benore, Marilee (2009). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. United States: Wiley. pp. 408–10. ISBN 978-0-470-08766-4.
  6. ^ Cheng S, Fockler C, Barnes WM, Higuchi R (June 1994). «Effective amplification of long targets from cloned inserts and human genomic DNA». Proceedings of the National Academy of Sciences of the United States of America. 91 (12): 5695–99. Bibcode:1994PNAS…91.5695C. doi:10.1073/pnas.91.12.5695. PMC 44063. PMID 8202550.
  7. ^ Carr AC, Moore SD (2012). Lucia A (ed.). «Robust quantification of polymerase chain reactions using global fitting». PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO…737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
  8. ^ a b Joseph Sambrook & David W. Russel (2001). Molecular Cloning: A Laboratory Manual (third ed.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. ISBN 978-0-879-69576-7. Chapter 8: In vitro Amplification of DNA by the Polymerase Chain Reaction
  9. ^ «Polymerase Chain Reaction (PCR)». National Center for Biotechnology Information, U.S. National Library of Medicine.
  10. ^ «PCR». Genetic Science Learning Center, University of Utah.
  11. ^ Pavlov AR, Pavlova NV, Kozyavkin SA, Slesarev AI (May 2004). «Recent developments in the optimization of thermostable DNA polymerases for efficient applications». Trends in Biotechnology. 22 (5): 253–60. doi:10.1016/j.tibtech.2004.02.011. PMID 15109812.
  12. ^ Rychlik W, Spencer WJ, Rhoads RE (November 1990). «Optimization of the annealing temperature for DNA amplification in vitro». Nucleic Acids Research. 18 (21): 6409–12. doi:10.1093/nar/18.21.6409. PMC 332522. PMID 2243783.
  13. ^ a b Sharkey DJ, Scalice ER, Christy KG, Atwood SM, Daiss JL (May 1994). «Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction». Bio/Technology. 12 (5): 506–09. doi:10.1038/nbt0594-506. PMID 7764710. S2CID 2885453.
  14. ^ a b Chien A, Edgar DB, Trela JM (September 1976). «Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus». Journal of Bacteriology. 127 (3): 1550–57. doi:10.1128/jb.127.3.1550-1557.1976. PMC 232952. PMID 8432.
  15. ^ a b Lawyer FC, Stoffel S, Saiki RK, Chang SY, Landre PA, Abramson RD, Gelfand DH (May 1993). «High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity». PCR Methods and Applications. 2 (4): 275–87. doi:10.1101/gr.2.4.275. PMID 8324500.
  16. ^ a b c d Schochetman G, Ou CY, Jones WK (December 1988). «Polymerase chain reaction». The Journal of Infectious Diseases. 158 (6): 1154–57. doi:10.1093/infdis/158.6.1154. JSTOR 30137034. PMID 2461996.
  17. ^ Borman, Jon; Schuster, David; Li, Wu-bo; Jessee, Joel; Rashtchian, Ayoub (2000). «PCR from problematic templates» (PDF). Focus. 22 (1): 10. Archived from the original (PDF) on 7 March 2017.
  18. ^ Bogetto, Prachi; Waidne, Lisa; Anderson, Holly (2000). «Helpful tips for PCR» (PDF). Focus. 22 (1): 12. Archived from the original (PDF) on 7 March 2017.
  19. ^ Biolabs, New England. «Q5® High-Fidelity DNA Polymerase | NEB». www.neb.com. Retrieved 4 December 2021.
  20. ^ Sze, Marc A.; Schloss, Patrick D. (2019). «The Impact of DNA Polymerase and Number of Rounds of Amplification in PCR on 16S rRNA Gene Sequence Data». mSphere. 4 (3): e00163–19. doi:10.1128/mSphere.00163-19. PMC 6531881. PMID 31118299.
  21. ^ Sarkar G, Kapelner S, Sommer SS (December 1990). «Formamide can dramatically improve the specificity of PCR». Nucleic Acids Research. 18 (24): 7465. doi:10.1093/nar/18.24.7465. PMC 332902. PMID 2259646.
  22. ^ «Electronic PCR». NCBI – National Center for Biotechnology Information. Retrieved 13 March 2012.
  23. ^ Pavlov AR, Pavlova NV, Kozyavkin SA, Slesarev AI (2006). «Thermostable DNA Polymerases for a Wide Spectrum of Applications: Comparison of a Robust Hybrid TopoTaq to other enzymes». In Kieleczawa J (ed.). DNA Sequencing II: Optimizing Preparation and Cleanup. Jones & Bartlett. pp. 241–57. ISBN 978-0-7637-3383-4.
  24. ^ Pombert JF, Sistek V, Boissinot M, Frenette M (October 2009). «Evolutionary relationships among salivarius streptococci as inferred from multilocus phylogenies based on 16S rRNA-encoding, recA, secA, and secY gene sequences». BMC Microbiology. 9: 232. doi:10.1186/1471-2180-9-232. PMC 2777182. PMID 19878555.
  25. ^ «Chemical Synthesis, Sequencing, and Amplification of DNA (class notes on MBB/BIO 343)». Arizona State University. Archived from the original on 9 October 1997. Retrieved 29 October 2007.
  26. ^ Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. (April 2009). «The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments» (PDF). Clinical Chemistry. 55 (4): 611–22. doi:10.1373/clinchem.2008.112797. PMID 19246619.
  27. ^ a b c d Garibyan L, Avashia N (March 2013). «Polymerase chain reaction». The Journal of Investigative Dermatology. 133 (3): 1–4. doi:10.1038/jid.2013.1. PMC 4102308. PMID 23399825.
  28. ^ Schnell, S.; Mendoza, C. (October 1997). «Theoretical Description of the Polymerase Chain Reaction». Journal of Theoretical Biology. 188 (3): 313–18. Bibcode:1997JThBi.188..313S. doi:10.1006/jtbi.1997.0473. PMID 9344735.
  29. ^ Schnell, S.; Mendoza, C. (21 February 1997). «Enzymological Considerations for the Theoretical Description of the Quantitative Competitive Polymerase Chain Reaction (QC-PCR)». Journal of Theoretical Biology. 184 (4): 433–40. Bibcode:1997JThBi.184..433S. doi:10.1006/jtbi.1996.0283. ISSN 0022-5193. PMID 9082073.
  30. ^ Becker, Sven; Böger, Peter; Oehlmann, Ralfh; Ernst, Anneliese (1 November 2000). «PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities». Applied and Environmental Microbiology. 66 (11): 4945–53. Bibcode:2000ApEnM..66.4945B. doi:10.1128/AEM.66.11.4945-4953.2000. ISSN 1098-5336. PMC 92404. PMID 11055948.
  31. ^ Solomon, Anthony W.; Peeling, Rosanna W.; Foster, Allen; Mabey, David C. W. (1 October 2004). «Diagnosis and Assessment of Trachoma». Clinical Microbiology Reviews. 17 (4): 982–1011. doi:10.1128/CMR.17.4.982-1011.2004. ISSN 0893-8512. PMC 523557. PMID 15489358.
  32. ^ Ramzy, Reda M.R. (April 2002). «Recent advances in molecular diagnostic techniques for human lymphatic filariasis and their use in epidemiological research». Transactions of the Royal Society of Tropical Medicine and Hygiene. 96: S225–29. doi:10.1016/S0035-9203(02)90080-5. PMID 12055843.
  33. ^ Sachse, Konrad (2003). «Specificity and performance of diagnostic PCR assays». In Sachse, Konrad; Frey, Joachim (eds.). PCR Detection of Microbial Pathogens. Methods in Molecular Biology. Vol. 216. Totowa, New Jersey: Humana Press. pp. 3–29. doi:10.1385/1-59259-344-5:03. ISBN 978-1-59259-344-6. PMID 12512353.
  34. ^ Quill E (March 2008). «Medicine. Blood-matching goes genetic». Science. 319 (5869): 1478–79. doi:10.1126/science.319.5869.1478. PMID 18339916. S2CID 36945291.
  35. ^ Tomar, Rukam (2010). Molecular Markers and Plant Biotechnology. Pitman Pura, New Delhi: New India Publishing Agency. p. 188. ISBN 978-93-80235-25-7.
  36. ^ a b c Cai HY, Caswell JL, Prescott JF (March 2014). «Nonculture molecular techniques for diagnosis of bacterial disease in animals: a diagnostic laboratory perspective». Veterinary Pathology. 51 (2): 341–50. doi:10.1177/0300985813511132. PMID 24569613.
  37. ^ Salis AD (2009). «Applications in Clinical Microbiology». Real-Time PCR: Current Technology and Applications. Caister Academic Press. ISBN 978-1-904455-39-4.
  38. ^ Kwok S, Mack DH, Mullis KB, Poiesz B, Ehrlich G, Blair D, et al. (May 1987). «Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection». Journal of Virology. 61 (5): 1690–94. doi:10.1128/jvi.61.5.1690-1694.1987. PMC 254157. PMID 2437321.
  39. ^ «Coronavirus: il viaggio dei test». Istituto Superiore di Sanità.
  40. ^ Finger, Horst; von Koenig, Carl Heinz Wirsing (1996). Baron, Samuel (ed.). Medical Microbiology (4th ed.). Galveston, TX: University of Texas Medical Branch at Galveston. ISBN 978-0-9631172-1-2. PMID 21413270.
  41. ^ Yeh, Sylvia H.; Mink, ChrisAnna M. (2012). «Bordetella pertussis and Pertussis (Whooping Cough)». Netter’s Infectious Diseases. Netter’s Infectious Diseases. pp. 11–14. doi:10.1016/B978-1-4377-0126-5.00003-3. ISBN 978-1-4377-0126-5.
  42. ^ Alonso A, Martín P, Albarrán C, García P, García O, de Simón LF, et al. (January 2004). «Real-Time PCR Designs to Estimate Nuclear and Mitochondrial DNA Copy Number in Forensic and Ancient DNA Studies». Forensic Science International. 139 (2–3): 141–49. doi:10.1016/j.forsciint.2003.10.008. PMID 15040907.
  43. ^ Boehnke M, Arnheim N, Li H, Collins FS (July 1989). «Fine-structure genetic mapping of human chromosomes using the polymerase chain reaction on single sperm: experimental design considerations». American Journal of Human Genetics. 45 (1): 21–32. PMC 1683385. PMID 2568090.
  44. ^ Zhou YH, Zhang XP, Ebright RH (November 1991). «Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase». Nucleic Acids Research. 19 (21): 6052. doi:10.1093/nar/19.21.6052. PMC 329070. PMID 1658751.
  45. ^ Stursberg, Stephanie (2021). «Setting up a PCR lab from scratch». INTEGRA Biosciences.{{cite web}}: CS1 maint: url-status (link)
  46. ^ Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, et al. (April 1989). «Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)». Nucleic Acids Research. 17 (7): 2503–16. doi:10.1093/nar/17.7.2503. PMC 317639. PMID 2785681.
  47. ^ Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (October 1995). «Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides». Gene. 164 (1): 49–53. doi:10.1016/0378-1119(95)00511-4. PMID 7590320.
  48. ^ Innis MA, Myambo KB, Gelfand DH, Brow MA (December 1988). «DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA». Proceedings of the National Academy of Sciences of the United States of America. 85 (24): 9436–40. Bibcode:1988PNAS…85.9436I. doi:10.1073/pnas.85.24.9436. PMC 282767. PMID 3200828.
  49. ^ Pierce KE, Wangh LJ (2007). Linear-after-the-exponential polymerase chain reaction and allied technologies Real-time detection strategies for rapid, reliable diagnosis from single cells. Methods in Molecular Medicine. Vol. 132. pp. 65–85. doi:10.1007/978-1-59745-298-4_7. ISBN 978-1-58829-578-1. PMID 17876077.
  50. ^ Krishnan M, Ugaz VM, Burns MA (October 2002). «PCR in a Rayleigh-Bénard convection cell». Science. 298 (5594): 793. doi:10.1126/science.298.5594.793. PMID 12399582.
  51. ^ Priye A, Hassan YA, Ugaz VM (November 2013). «Microscale chaotic advection enables robust convective DNA replication». Analytical Chemistry. 85 (21): 10536–41. doi:10.1021/ac402611s. PMID 24083802.
  52. ^ Schwartz JJ, Lee C, Shendure J (September 2012). «Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules». Nature Methods. 9 (9): 913–15. doi:10.1038/nmeth.2137. PMC 3433648. PMID 22886093.
  53. ^ Vincent M, Xu Y, Kong H (August 2004). «Helicase-dependent isothermal DNA amplification». EMBO Reports. 5 (8): 795–800. doi:10.1038/sj.embor.7400200. PMC 1249482. PMID 15247927.
  54. ^ Chou Q, Russell M, Birch DE, Raymond J, Bloch W (April 1992). «Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications». Nucleic Acids Research. 20 (7): 1717–23. doi:10.1093/nar/20.7.1717. PMC 312262. PMID 1579465.
  55. ^ Kellogg DE, Rybalkin I, Chen S, Mukhamedova N, Vlasik T, Siebert PD, Chenchik A (June 1994). «TaqStart Antibody: «hot start» PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase». BioTechniques. 16 (6): 1134–37. PMID 8074881.
  56. ^ San Millán RM, Martínez-Ballesteros I, Rementeria A, Garaizar J, Bikandi J (December 2013). «Online exercise for the design and simulation of PCR and PCR-RFLP experiments». BMC Research Notes. 6: 513. doi:10.1186/1756-0500-6-513. PMC 4029544. PMID 24314313.
  57. ^ Zietkiewicz E, Rafalski A, Labuda D (March 1994). «Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification». Genomics. 20 (2): 176–83. doi:10.1006/geno.1994.1151. PMID 8020964.
  58. ^ Ochman H, Gerber AS, Hartl DL (November 1988). «Genetic applications of an inverse polymerase chain reaction». Genetics. 120 (3): 621–23. doi:10.1093/genetics/120.3.621. PMC 1203539. PMID 2852134.
  59. ^ Mueller PR, Wold B (November 1989). «In vivo footprinting of a muscle specific enhancer by ligation mediated PCR». Science. 246 (4931): 780–86. Bibcode:1989Sci…246..780M. doi:10.1126/science.2814500. PMID 2814500.
  60. ^ Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB (September 1996). «Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands». Proceedings of the National Academy of Sciences of the United States of America. 93 (18): 9821–26. Bibcode:1996PNAS…93.9821H. doi:10.1073/pnas.93.18.9821. PMC 38513. PMID 8790415.
  61. ^ Isenbarger TA, Finney M, Ríos-Velázquez C, Handelsman J, Ruvkun G (February 2008). «Miniprimer PCR, a new lens for viewing the microbial world». Applied and Environmental Microbiology. 74 (3): 840–49. Bibcode:2008ApEnM..74..840I. doi:10.1128/AEM.01933-07. PMC 2227730. PMID 18083877.
  62. ^ Shen C, Yang W, Ji Q, Maki H, Dong A, Zhang Z (November 2009). «NanoPCR observation: different levels of DNA replication fidelity in nanoparticle-enhanced polymerase chain reactions». Nanotechnology. 20 (45): 455103. Bibcode:2009Nanot..20S5103S. doi:10.1088/0957-4484/20/45/455103. PMID 19822925. S2CID 3393115.
  63. ^ Shen, Cenchao (2013). «An Overview of Nanoparticle-Assisted Polymerase Chain Reaction Technology». An Overview of Nanoparticle‐Assisted Polymerase Chain Reaction Technology. US: Wiley-Blackwell Publishing Ltd. pp. 97–106. doi:10.1002/9781118451915.ch5. ISBN 978-1-118-45191-5.
  64. ^ Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (April 1989). «Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension». Gene. 77 (1): 61–68. doi:10.1016/0378-1119(89)90359-4. PMID 2744488.
  65. ^ Moller, Simon (2006). PCR: The Basics. US: Taylor & Francis Group. p. 144. ISBN 978-0-415-35547-6.
  66. ^ David F, Turlotte E (November 1998). «[A method of isothermal gene amplification]» [An Isothermal Amplification Method]. Comptes Rendus de l’Académie des Sciences, Série III. 321 (11): 909–14. Bibcode:1998CRASG.321..909D. doi:10.1016/S0764-4469(99)80005-5. PMID 9879470.
  67. ^ Fabrice David (September–October 2002). «Utiliser les propriétés topologiques de l’ADN: une nouvelle arme contre les agents pathogènes» (PDF). Fusion. Archived from the original (PDF) on 28 November 2007.(in French)
  68. ^ Dobosy JR, Rose SD, Beltz KR, Rupp SM, Powers KM, Behlke MA, Walder JA (August 2011). «RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers». BMC Biotechnology. 11: 80. doi:10.1186/1472-6750-11-80. PMC 3224242. PMID 21831278.
  69. ^ Shyamala, V.; Ferro-Luzzi, Ames G. (1993). Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. Methods in Molecular Biology. Vol. 15. pp. 339–48. doi:10.1385/0-89603-244-2:339. ISBN 978-0-89603-244-6. PMID 21400290.
  70. ^ Bing DH, Boles C, Rehman FN, Audeh M, Belmarsh M, Kelley B, Adams CP (1996). «Bridge amplification: a solid phase PCR system for the amplification and detection of allelic differences in single copy genes». Genetic Identity Conference Proceedings, Seventh International Symposium on Human Identification. Archived from the original on 7 May 2001.
  71. ^ Khan Z, Poetter K, Park DJ (April 2008). «Enhanced solid phase PCR: mechanisms to increase priming by solid support primers». Analytical Biochemistry. 375 (2): 391–93. doi:10.1016/j.ab.2008.01.021. PMID 18267099.
  72. ^ Raoult D, Aboudharam G, Crubézy E, Larrouy G, Ludes B, Drancourt M (November 2000). «Molecular identification by «suicide PCR» of Yersinia pestis as the agent of medieval black death». Proceedings of the National Academy of Sciences of the United States of America. 97 (23): 12800–03. Bibcode:2000PNAS…9712800R. doi:10.1073/pnas.220225197. PMC 18844. PMID 11058154.
  73. ^ Liu YG, Whittier RF (February 1995). «Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking». Genomics. 25 (3): 674–81. doi:10.1016/0888-7543(95)80010-J. PMID 7759102.
  74. ^ Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (July 1991). «‘Touchdown’ PCR to circumvent spurious priming during gene amplification». Nucleic Acids Research. 19 (14): 4008. doi:10.1093/nar/19.14.4008. PMC 328507. PMID 1861999.
  75. ^ Myrick KV, Gelbart WM (February 2002). «Universal Fast Walking for direct and versatile determination of flanking sequence». Gene. 284 (1–2): 125–31. doi:10.1016/S0378-1119(02)00384-0. PMID 11891053.
  76. ^ «Full Text – LaNe RAGE: a new tool for genomic DNA flanking sequence determination». www.ejbiotechnology.info.
  77. ^ Park DJ (January 2005). «A new 5′ terminal murine GAPDH exon identified using 5’RACE LaNe». Molecular Biotechnology. 29 (1): 39–46. doi:10.1385/MB:29:1:39. PMID 15668518. S2CID 45702164.
  78. ^ Park DJ (April 2004). «3′ RACE LaNe: a simple and rapid fully nested PCR method to determine 3′-terminal cDNA sequence». BioTechniques. 36 (4): 586–88, 590. doi:10.2144/04364BM04. PMID 15088375.
  79. ^ «Key ingredient in coronavirus tests comes from Yellowstone’s lakes». Science. 31 March 2020. Retrieved 13 May 2020.
  80. ^ Kleppe K, Ohtsuka E, Kleppe R, Molineux I, Khorana HG (March 1971). «Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA’s as catalyzed by DNA polymerases». Journal of Molecular Biology. 56 (2): 341–61. doi:10.1016/0022-2836(71)90469-4. PMID 4927950.
  81. ^ Rabinow, Paul (1996). Making PCR: A Story of Biotechnology. Chicago: University of Chicago Press. ISBN 978-0-226-70146-2.
  82. ^ Mullis, Kary (1998). Dancing Naked in the Mind Field. New York: Pantheon Books. ISBN 978-0-679-44255-4.
  83. ^ Mullis KB (April 1990). «The unusual origin of the polymerase chain reaction». Scientific American. 262 (4): 56–61, 64–65. Bibcode:1990SciAm.262d..56M. doi:10.1038/scientificamerican0490-56. PMID 2315679.
  84. ^ Patidar M, Agrawal S, Parveen F, Khare P (2015). «Molecular insights of saliva in solving paternity dispute». Journal of Forensic Dental Sciences. 7 (1): 76–79. doi:10.4103/0975-1475.150325. PMC 4330625. PMID 25709326.
  85. ^ Nichols D, Barker E (2016). «Psychedelics». Pharmacological Reviews. 68 (2): 264–355. doi:10.1124/pr.115.011478. PMC 4813425. PMID 26841800.
  86. ^ «The Nobel Prize in Chemistry 1993». NobelPrize.org.
  87. ^ «Citations for Chemical Breakthrough Awards 2017 Awardees». Division of the History of Chemistry. Retrieved 12 March 2018.
  88. ^ Fore, J. Jr.; Wiechers, I. R.; Cook-Deegan, R. (3 July 2006). «The effects of business practices, licensing, and intellectual property on development and dissemination of the polymerase chain reaction: case study». Journal of Biomedical Discovery and Collaboration. 1: 7. doi:10.1186/1747-5333-1-7. PMC 1523369. PMID 16817955.
  89. ^ «Advice on How to Survive the Taq Wars». GEN Genetic Engineering News – Biobusiness Channel. 26 (9). 1 May 2006.

External links[edit]

  • US Patent for PCR
  • What is PCR plateau effect? YouTube tutorial video
  • History of the Polymerase Chain Reaction from the Smithsonian Institution Archives

Примеры из текстов

Авторами показана возможность выполнения ПЦР в описанном устройстве при времени цикла равном 6,6 секунд.

The authors demonstrated the possibility of PCR in the described device with the cycle time of 6.6 seconds.

Для этого используется метод, так называемой, асимметричной ПЦР.

For this, the so-called method of asymmetrical PCR is used.

В последние годы все более широкое применение находит так называемая ПЦР в реальном времени (ПЦР-РВ), когда детекция целевого продукта ведется прямо в ходе амплификации с помощью специальных ДНК-амплификаторов, оснащенных оптическим модулем.

In recent years, the real time PCR is being used more and more, wherein the detection of the target product is carried out directly during amplification with the aid of special DNA thermocyclers equipped with an optical module.

Для проведения ПЦР анализа ввод раствора образца согласно п.2 настоящего примера, осуществлялся в реакционные зоны микрочипа аналогично примеру 1.

For the PCR analysis the solution of the sample according to item 2 of the present example was introduced into the reaction zones of the microchip in the way similar to example 1.

ПЦР-продукты, полученные на второй стадии амплификации, гибридизуют на биочипе с иммобилизованными дифференцирующими олигонуклеотидами, комплементарными участкам консенсусных последовательностей генотипов и подтипов фрагмента области NS 5b.

PCR-products from a second amplification step are hybridized on a biochip with immobilized differentiating oligonucleotides complementary to the consensus sequences of genotypes and subtypes of an NS5B region fragment.

В результате были получены ПЦР PB кривые (данные не приведены), из которых было видно, что в пробах, содержащих искомую ДНК, наблюдается увеличение сигнала флуоресценции, а в пробах, не содержащих ДНК, увеличения сигнала флуоресценции не происходит.

As result rt-PCR curves were obtained (no data are available) that show that the samples containing the target DNA exhibit increased fluorescence signal while the samples that do not contain the target DNA the fluorescence signal does not increase.

После своего появления в середине 80-х годов двадцатого столетия ПЦР, по крайней мере, по масштабу применимости довольно быстро стала фактически методом N°l в медико-биологических науках и в диагностике.

After it appeared in the middle of the eighties in the twentieth century, PCR, at least in respect to the scale of its use, rather rapidly became the actual method No. 1 in the biomedical sciences and in diagnostics.

Однако во вложенной ПЦР роста числа мест отжига праймера не происходит и каждый из них (внешний и внутренний) «работает» практически независимо от другого.

However, in the nested PCR, an increase in the number of places of annealing the primer does not take place and each of them (external and internal) “works” independently of the other.

Предпочтительно, чтобы недостающие компоненты ПЦР были внесены в реакционные зоны до ввода проб.

It is preferred that the remaining PCR components be introduced into the reaction zones before the sample introduction.

В результате за 1,5 часа реакции наработался специфичный ПЦР-продукт, количество которого оказалось достаточным, чтобы его увидеть с помощью агарозного гель- электрофореза.

As a result a specific PCR product was produced in 1.5 hours of the reaction, the amount of which product turned out to be sufficient for it to be seen with use of agarose gel electrophoresis.

В этом же патенте раскрываются также способы, позволяющие создавать защитное покрытие поверхностей микрореакторов, в которых проводится ПЦР.

The same patent also displays some methods that allow PCR microreactor surface passivation.

Детекцию образовавшегося комплекса (стадия б) осуществляют флуориметрически, хемилюминометрически, масс-спектрометрически или путем проведения иммуно- ПЦР с последующей регистрацией результатов ПЦР на том же микрочипе.

The detection of the formed complex (step b)) is carried out fluorimetrically, chemiluminometrically, mass- spectrometrically or by carrying out immuno-PCR with subsequent registration of the PCR results on the same microchip.

Еще более предпочтительно, чтобы до ввода проб компоненты ПЦР были внесены в реакционные зоны в высушенном виде.

It is even more advantageous that the PCR components be introduced into the reaction zones in dried form before introduction of the samples.

Анализ проводили в 96-луночной плашке, результат прохождения ПЦР далее оценивали по данным электрофореза.

Assay was carried out in a 96-cell plate, the PCR result was further evaluated by electrophoresis data.

В дальнейшем были получены еще два отрицательных анализа ПЦР в течение двух недель, после чего введение реаферона было прекращено.

Further on, two more negative polymerase chain reaction tests were obtained within two weeks and then reaferon administration was stopped.

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